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AB109023

Anti-Munc18-1 抗体 [EPR4849]

Anti-Munc18-1 antibody [EPR4849]

5

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal Munc18-1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

別名を表示する

UNC18A, STXBP1, Syntaxin-binding protein 1, MUNC18-1, N-Sec1, Protein unc-18 homolog 1, Protein unc-18 homolog A, p67, Unc18-1, Unc-18A

2 Images
Western blot - Anti-Munc18-1 antibody [EPR4849] (AB109023)
  • WB

Unknown

Western blot - Anti-Munc18-1 antibody [EPR4849] (AB109023)

All lanes:

Western blot - Anti-Munc18-1 antibody [EPR4849] (ab109023) at 1/1000 dilution

Lane 1:

SH SY5Y cell lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Predicted band size: 68 kDa

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Western blot - Anti-Munc18-1 antibody [EPR4849] (AB109023)
  • WB

Supplier Data

Western blot - Anti-Munc18-1 antibody [EPR4849] (AB109023)

ab109023 was shown to react with STXBP1 in wild-type U-87 MG cells in Western blot with loss of signal observed in STXBP1 knockout cell line ab322392. Wild-type U-87 MG and STXBP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab109023 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Munc18-1 antibody [EPR4849] (ab109023) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG lysate at 40 µg

Lane 2:

STXBP1 knock-out U-87 MG lysate at 40 µg

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Munc18-1 antibody [EPR4849] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4849

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB183484

Anti-Munc18-1 (phospho S241) antibody

Western blot - Anti-Munc18-1 (phospho S241) antibody (AB183484)

  • 0
  • 0
Primary Antibodies

AB155020

Anti-Munc18-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Munc18-1 antibody (AB155020)

  • 0
  • 0
Primary Antibodies

AB138687

Anti-Munc18-1 (phospho S313) antibody

Western blot - Anti-Munc18-1 (phospho S313) antibody (AB138687)

  • 0
  • 0
Primary Antibodies

AB3451

Anti-Munc18-1 antibody

Immunocytochemistry - Anti-Munc18-1 antibody (AB3451)

  • 5
  • 2
Primary Antibodies

AB264385

Anti-Munc18-1 antibody

Immunoprecipitation - Anti-Munc18-1 antibody (AB264385)

  • 0
  • 0
Primary Antibodies

AB124920

Anti-Munc18-1 antibody [EPR4850]

KO Validated|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Munc18-1 antibody [EPR4850] (AB124920)

  • 4
  • 1

組合せ製品

Assay Kits

AB314357

Human Munc18-1 ELISA Kit

Sandwich ELISA - Human Munc18-1 ELISA Kit (AB314357)

  • 0
  • 0
Primary Antibodies

AB181869

Anti-VAMP2 antibody [EPR12790]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)

  • 5
  • 1
Primary Antibodies

AB109105

Anti-SNAP25 antibody [EPR3275]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (AB109105)

  • 5
  • 3
Primary Antibodies

AB183484

Anti-Munc18-1 (phospho S241) antibody

Western blot - Anti-Munc18-1 (phospho S241) antibody (AB183484)

  • 0
  • 0
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 75
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Munc18-1 also known as Syntaxin-binding protein 1 is an important protein involved in the regulation of neurotransmitter release at synapses. It has a molecular mass of approximately 67 kDa. This protein is highly expressed in the brain where it plays a significant role in synaptic vesicle exocytosis. Munc18-1 interacts with components of the SNARE complex facilitating the docking and fusion of synaptic vesicles with the presynaptic membrane.
Biological function summary

This protein is important for the proper release of neurotransmitters into the synaptic cleft. Munc18-1 functions as a part of the neuronal exocytosis complex where it regulates the interaction with syntaxin-1 a core SNARE protein. This protein-protein interaction stabilizes an intermediate state necessary for vesicle priming and subsequent fusion ensuring efficient synaptic transmission. By controlling vesicle movement Munc18-1 influences communication between neurons.

Pathways

Munc18-1 integrates into the neurotransmitter release pathway which is central to synaptic function. It closely associates with the SNARE complex involving proteins such as SNAP-25 and synaptobrevin. Another important pathway is the synaptic vesicle cycle where Munc18-1 plays a role in vesicle docking and priming stages. Through these interactions it ensures that synaptic vesicles are ready and able to release their contents upon stimulation.

Alterations in Munc18-1 expression or function connect to neurological conditions like epilepsy and intellectual disability. In epilepsy dysregulation of neurotransmitter release due to modified Munc18-1 function disrupts synaptic communication leading to seizure activity. In the context of these disorders Munc18-1 interacts functionally with proteins such as VAMP2 contributing to the pathological state when disrupted. These interactions highlight the importance of Munc18-1 in maintaining normal neurological function.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1 : 1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. Involved in the release of neurotransmitters from neurons through interacting with SNARE complex component STX1A and mediating the assembly of the SNARE complex at synaptic membranes (By similarity). May play a role in determining the specificity of intracellular fusion reactions.
See full target information STXBP1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Stress (Amsterdam, Netherlands) 27:2312467 PubMed38557197

2024

Chronic adolescent stress alters GR-FKBP5 interactions in the hippocampus of adult female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney Rowson,Mandakh Bekhbat,Sean Kelly,Molly M Hyer,Samya Dyer,David Weinshenker,Gretchen Neigh

Prion 15:29-36 PubMed33590815

2021

STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system.

Applications

Unspecified application

Species

Unspecified reactive species

A V Chirinskaite,V A Siniukova,M E Velizhanina,J V Sopova,T A Belashova,S P Zadorsky

Biochemical pharmacology 173:113734 PubMed31811867

2019

Ageing enhances the shedding of splenocyte microvesicles with endothelial pro-senescent effect that is prevented by a short-term intake of omega-3 PUFA EPA:DHA 6:1.

Applications

Unspecified application

Species

Unspecified reactive species

A W Qureshi,R Altamimy,A El Habhab,H El Itawi,M A Farooq,F Zobairi,H Hasan,L Amoura,M Kassem,C Auger,V Schini-Kerth,F Toti
View all publications
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Abcam product promise

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