Anti-Munc18-1 抗体 (ab3451)
Key features and details
- Rabbit polyclonal to Munc18-1
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Munc18-1 antibody
Munc18-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Munc18-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human Munc18-1 aa 58-70.
Sequence:VEDINKRREPIPS
(Peptide available asab4982) -
ポジティブ･コントロール
- WB: Mouse and rat brain tissue lysates ICC: U-87 MG cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3451の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa.
By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract. |
| ICC |
Use a concentration of 2 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa. By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract. |
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ICC
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
組織特異性
Brain and spinal cord. Highly enriched in axons. -
関連疾患
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
配列類似性
Belongs to the STXBP/unc-18/SEC1 family. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 404293 Chicken
- Entrez Gene: 282378 Cow
- Entrez Gene: 6812 Human
- Entrez Gene: 20910 Mouse
- Entrez Gene: 25558 Rat
- Omim: 602926 Human
- SwissProt: Q6R748 Chicken
- SwissProt: P61763 Cow
see all -
別名
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
画像
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Western blot - Anti-Munc18-1 antibody (ab3451)Anti-Munc18-1 antibody (ab3451) at 2 µg/ml + Mouse brain tissue lysate at 30 µg
Secondary
Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution
Observed band size: 67 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-Munc18-1 antibody (ab3451)Immunocytochemistry analysis of 70% confluent log phase U-87 MG cells labeling Munc18-1 with ab3451 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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Western blot - Anti-Munc18-1 antibody (ab3451)All lanes : Anti-Munc18-1 antibody (ab3451) at 2 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution
Observed band size: 67 kDa why is the actual band size different from the predicted?Detected by chemiluminescence
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Western blot - Anti-Munc18-1 antibody (ab3451)This image is courtesy of an anonymous AbreviewAnti-Munc18-1 antibody (ab3451) at 1/5000 dilution + Mouse hippocampal homogenate at 5 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 67 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-Munc18-1 antibody (ab3451)Western blot of Munc-18 in rat brain homogenate immunoprecipitated with ab3451.
1. Extract only.
2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of ab3451, respectively.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab3451 は 13 報の論文で使用されています。
- Tang F et al. Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels. Neurochem Res 48:791-803 (2023). PubMed: 36335177
- Lu Z et al. Base-edited cynomolgus monkeys mimic core symptoms of STXBP1 encephalopathy. Mol Ther 30:2163-2175 (2022). PubMed: 35283272
- Wang CC et al. Presynaptic Short-Term Plasticity Persists in the Absence of PKC Phosphorylation of Munc18-1. J Neurosci 41:7329-7339 (2021). PubMed: 34290081
- Ishii C et al. CAPS1 is involved in hippocampal synaptic plasticity and hippocampus-associated learning. Sci Rep 11:8656 (2021). PubMed: 33883618
- Di Marco Vieira B et al. Extracellular Alpha-Synuclein Promotes a Neuroinhibitory Secretory Phenotype in Astrocytes. Life (Basel) 10:N/A (2020). PubMed: 32911644