Anti-Munc18-1 抗体 (ab155020)
Key features and details
- Rabbit polyclonal to Munc18-1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Munc18-1 antibody
Munc18-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Munc18-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Xenopus laevis
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免疫原
Recombinant fragment corresponding to a region within amino acids 354-574 of Human Munc18.
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ポジティブ･コントロール
- 293T, A431, Jurkat, Raji and mouse brain lysates; A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155020の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
組織特異性
Brain and spinal cord. Highly enriched in axons. -
関連疾患
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
配列類似性
Belongs to the STXBP/unc-18/SEC1 family. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 404293 Chicken
- Entrez Gene: 282378 Cow
- Entrez Gene: 6812 Human
- Entrez Gene: 20910 Mouse
- Entrez Gene: 25558 Rat
- Entrez Gene: 399128 Xenopus laevis
- Omim: 602926 Human
- SwissProt: Q6R748 Chicken
see all -
別名
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
画像
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Western blot - Anti-Munc18-1 antibody (ab155020)All lanes : Anti-Munc18-1 antibody (ab155020) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE -
Western blot - Anti-Munc18-1 antibody (ab155020)Anti-Munc18-1 antibody (ab155020) at 1/10000 dilution + mouse brain whole cell lysate at 20 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Munc18-1 antibody (ab155020)Confocal immunofluorescent analysis of methanol-fixed A431 cells labeling Munc18 with ab155020 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody at 1/2000 dilution (red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab155020 は論文での使用が確認できていません。