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AB109113

Anti-Munc 13-4 抗体 [EPR4914]

Anti-Munc 13-4 antibody [EPR4914]

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(3 Publications)

Rabbit Recombinant Monoclonal Munc 13-4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 3 publications.

別名を表示する

Protein unc-13 homolog D, Munc13-4, UNC13D

2 Images
Western blot - Anti-Munc 13-4 antibody [EPR4914] (AB109113)
  • WB

Unknown

Western blot - Anti-Munc 13-4 antibody [EPR4914] (AB109113)

All lanes:

Western blot - Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Molt-4 cell lysate at 10 µg

Lane 5:

RAW264.7 cell lysate at 10 µg

Predicted band size: 123 kDa

false

Flow Cytometry (Intracellular) - Anti-Munc 13-4 antibody [EPR4914] (AB109113)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Munc 13-4 antibody [EPR4914] (AB109113)

Intracellular flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.

関連する標識済み抗体及び組成の異なる製品 (5)

  • Carrier free

    Anti-Munc 13-4 antibody [EPR4914] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Munc 13-4 antibody [EPR4914]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Munc 13-4 antibody [EPR4914]

  • 578 PE

    PE Anti-Munc 13-4 antibody [EPR4914]

  • 660 APC

    APC Anti-Munc 13-4 antibody [EPR4914]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4914

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Munc13-4 also known as UNC13D acts as a critical mediator in the fusion of vesicles particularly in the exocytosis of cytotoxic granules in immune cells. This protein weighs approximately 120 kDa and resides in various immune cell types such as cytotoxic T lymphocytes and natural killer cells. Munc13-4 exists in the cytosol and relocates to the cell membrane upon activation supporting its role in exocytosis.
Biological function summary

Munc13-4 serves an essential function in immune response by facilitating granule-mediated cytotoxicity. This protein does not operate alone; it acts within a complex network with other members of the Unc13 protein family. The synergy between these proteins ensures the precise docking and priming of secretory vesicles aiding in effective immune responses against infected or malignant cells.

Pathways

Munc13-4 participates in the granule exocytosis pathway a central component of immune system functionality. Within this pathway Munc13-4 acts alongside proteins such as Syntaxin 11 and Rab27a influencing the regulation and release of cytolytic granules. Another vital pathway involving Munc13-4 is the calcium-dependent signaling pathway which modulates its activity and highlights its exocytotic role.

Defects in Munc13-4 have a strong association with familial hemophagocytic lymphohistiocytosis type 3 (FHL3) a severe immunological disorder. The impaired function of Munc13-4 results in defective cytotoxic granule exocytosis leading to improper immune regulation. Additionally connections exist between Munc13-4 dysfunction and systemic lupus erythematosus (SLE) which implicates proteins such as Perforin and Rab27a in altered immune responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
See full target information UNC13D
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 134: PubMed33093239

2020

Unc-13 homolog D mediates an antiviral effect of the chromosome 19 microRNA cluster miR-517a.

Applications

Unspecified application

Species

Unspecified reactive species

Kamil Krawczynski,Yingshi Ouyang,Jean-Francois Mouillet,Tianjiao Chu,Carolyn B Coyne,Yoel Sadovsky

Human gene therapy 31:626-638 PubMed32253931

2020

Lentiviral Gene Therapy for Familial Hemophagocytic Lymphohistiocytosis Type 3, Caused by Genetic Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Takushi,Na Yoon Paik,Andrew Fedanov,Chengyu Prince,Christopher B Doering,H Trent Spencer,Shanmuganathan Chandrakasan

Molecular medicine reports 18:1015-1024 PubMed29767240

2018

Munc13‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Xu,Jia Zhou,Xiang-Dong Zhou,Qi Li,Juliy M Perelman,Victor P Kolosov
View all publications
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