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Anti-Mumps nucleoprotein 抗体 [8H4] (ab9876)

Submit a review Q&A (1)References (8)
Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (ab9876)

    Key features and details

    • Mouse monoclonal [8H4] to Mumps nucleoprotein
    • Suitable for: ICC/IF
    • Isotype: IgG2b

    製品の概要

    • 製品名

      Anti-Mumps nucleoprotein antibody [8H4]
      Mumps nucleoprotein 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [8H4] to Mumps nucleoprotein

    • 由来種

      Mouse

    • 特異性

      Recognises recombinant yeast-derived mumps NP. Recognises native NP in mumps virus infected Vero cells. This antibody has been tested on 3 different mumps virus strains isolated in the UK: 55799-98, SJ801-95 and Gloucester.

    • アプリケーション

      適用あり: ICC/IFmore details

    • 種交差性

      交差種: Mumps virus

    • 免疫原

      Full length protein corresponding to Mumps nucleoprotein aa 1 to the C-terminus.
      Database link: AAG37826.1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 6.80
      Preservative: 0.1% Sodium azide
      Constituent: 15% Fetal Bovine Serum (FBS)
    • Concentration information loading...

    • 精製度

      Tissue culture supernatant

    • ポリ/モノ

      モノクローナル

    • クローン名

      8H4

    • ミエローマ

      NS0/1

    • アイソタイプ

      IgG2b

    • 軽鎖の種類

      unknown

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9876の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF
    1/1 - 1/20.
    特記事項
    ICC/IF
    1/1 - 1/20.

    ターゲット情報

    • 別名

      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (ab9876)
      Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (ab9876)

      Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4.



      Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4.

    参考文献 (8)

    ab9876 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9876 は 8 報の論文で使用されています。

    • Zhang X  et al. Molecular mechanisms of stress-induced reactivation in mumps virus condensates. Cell 186:1877-1894.e27 (2023). PubMed: 37116470
    • Hao X  et al. Development of Improved Mumps Vaccine Candidates by Mutating Viral mRNA Cap Methyltransferase Sites in the Large Polymerase Protein. Virol Sin 36:521-536 (2021). PubMed: 33284397
    • Wu H  et al. Mumps virus infection disrupts blood-testis barrier through the induction of TNF-a in Sertoli cells. FASEB J 33:12528-12540 (2019). PubMed: 31450968
    • Wu H  et al. Mouse Testicular Cell Type-Specific Antiviral Response against Mumps Virus Replication. Front Immunol 8:117 (2017). PubMed: 28239382
    • Jiang Q  et al. C-X-C motif chemokine ligand 10 produced by mouse Sertoli cells in response to mumps virus infection induces male germ cell apoptosis. Cell Death Dis 8:e3146 (2017). Mouse . PubMed: 29072682
    • Wu H  et al. Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells. Sci Rep 6:19507 (2016). PubMed: 26776505
    • Ma TM  et al. Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate-a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk. J Biol Chem 289:29631-41 (2014). WB . PubMed: 25164819
    • Samuel D  et al. High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol 66:123-30 (2002). PubMed: 11748668

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Please provide details of the stains of mumps virus that Abcam mumps antibodies have been tested on.

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    Answer

    Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

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