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AB9876

Anti-Mumps nucleoprotein 抗体 [8H4]

Anti-Mumps nucleoprotein antibody [8H4]

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(8 Publications)

Mouse Monoclonal NCAP antibody. Suitable for ICC/IF and reacts with Mumps orthorubulavirus samples. Cited in 8 publications.

別名を表示する

NP, Nucleocapsid protein, Nucleoprotein

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (AB9876)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (AB9876)

Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4. Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

8H4

アイソタイプ

IgG2b

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Mumps orthorubulavirus

アプリケーション

ICC/IF

applications

特異性

Recognises recombinant yeast-derived mumps NP. Recognises native NP in mumps virus infected Vero cells. This antibody has been tested on 3 different mumps virus strains isolated in the UK: 55799-98, SJ801-95 and Gloucester.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mumps orthorubulavirus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1 - 1/20", "ICCIF-species-notes": "<p></p>" } } }

製品の詳細

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 6.8 - 7.2 Preservative: 0.1% Sodium azide Constituents: 15% Fetal Bovine Serum (FBS)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The mumps nucleoprotein sometimes referred to as N protein plays an important role in the replication and transcription of the mumps virus acting as a structural element in the formation of viral ribonucleoprotein (RNP) complexes. The nucleoprotein is a large protein with a molecular mass of approximately 60 kDa and is expressed in cells that are infected by the mumps virus. It facilitates encapsidation of viral RNA and ensures the stability of the viral genome which is essential for virus survival and propagation.
Biological function summary

The mumps nucleoprotein functions as a part of the mumps virus replication complex. It interacts with other viral proteins such as the phosphoprotein (P protein) and the large protein (L protein) to initiate and regulate the replication of viral RNA. These interactions are critical for the stabilization of the entire complex ensuring the efficient production of new viral particles within host cells. The nucleoprotein is primarily located in the cytoplasm of infected cells where it associates with newly synthesized viral RNA strands.

Pathways

The mumps nucleoprotein is involved in the viral replication and transcription pathways specific to the mumps virus. These pathways facilitate viral spread and infection contributing to the virulence of the virus. The nucleoprotein works closely with L and P proteins which serve vital roles in the RNA polymerase complex guiding the synthesis of viral RNA. These interactions are part of the broader negative-strand RNA virus replication pathway shared by other viruses in the Paramyxoviridae family.

The mumps nucleoprotein is directly associated with the mumps disease a contagious viral infection that can lead to severe complications such as encephalitis and orchitis. Antibodies targeting the nucleoprotein can help in diagnosing mumps infections. The nucleoprotein also has implications in mumps-related complications where it interacts with immune system components. Research into this protein helps in understanding the immunological response to the mumps virus potentially informing vaccine development and antiviral therapies.

製品プロトコール

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:1877-1894.e27 PubMed37116470

2023

Molecular mechanisms of stress-induced reactivation in mumps virus condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Zhang,Sindhuja Sridharan,Ievgeniia Zagoriy,Christina Eugster Oegema,Cyan Ching,Tim Pflaesterer,Herman K H Fung,Isabelle Becher,Ina Poser,Christoph W Müller,Anthony A Hyman,Mikhail M Savitski,Julia Mahamid

Virologica Sinica 36:521-536 PubMed33284397

2020

Development of Improved Mumps Vaccine Candidates by Mutating Viral mRNA Cap Methyltransferase Sites in the Large Polymerase Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Hao,Yilong Wang,Mengying Zhu,Dongming Zhou,Rongxian Liu,Bin Wang,Yao-Wei Huang,Zhengyan Zhao

FASEB journal : official publication of the Federa 33:12528-12540 PubMed31450968

2019

Mumps virus infection disrupts blood-testis barrier through the induction of TNF-α in Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wu,Xing Jiang,Yunxiao Gao,Weihua Liu,Fei Wang,Maolei Gong,Ran Chen,Xiaoqin Yu,Wenjing Zhang,Bo Gao,Chengyi Song,Daishu Han

Cell death & disease 8:e3146 PubMed29072682

2017

C-X-C motif chemokine ligand 10 produced by mouse Sertoli cells in response to mumps virus infection induces male germ cell apoptosis.

Applications

Unspecified application

Species

Mouse

Qian Jiang,Fei Wang,Lili Shi,Xiang Zhao,Maolei Gong,Weihua Liu,Chengyi Song,Qihan Li,Yongmei Chen,Han Wu,Daishu Han

Frontiers in immunology 8:117 PubMed28239382

2017

Mouse Testicular Cell Type-Specific Antiviral Response against Mumps Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wu,Xiang Zhao,Fei Wang,Qian Jiang,Lili Shi,Maolei Gong,Weihua Liu,Bo Gao,Chengyi Song,Qihan Li,Yongmei Chen,Daishu Han

Scientific reports 6:19507 PubMed26776505

2016

Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wu,Lili Shi,Qing Wang,Lijing Cheng,Xiang Zhao,Qiaoyuan Chen,Qian Jiang,Min Feng,Qihan Li,Daishu Han

The Journal of biological chemistry 289:29631-41 PubMed25164819

2014

Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk.

Applications

WB

Species

Unspecified reactive species

Ting Martin Ma,Bindu D Paul,Chenglai Fu,Shaohui Hu,Heng Zhu,Seth Blackshaw,Herman Wolosker,Solomon H Snyder

Journal of medical virology 66:123-30 PubMed11748668

2001

High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology.

Applications

Unspecified application

Species

Unspecified reactive species

Dhanraj Samuel,Kestutis Sasnauskas,Li Jin,Stuart Beard,Aurelija Zvirbliene,Alma Gedvilaite,Bernard Cohen
View all publications

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