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AB9879

Anti-Mumps nucleoprotein 抗体 [5E3]

Anti-Mumps nucleoprotein antibody [5E3]

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(1 Publication)

Mouse Monoclonal NCAP antibody. Suitable for I-ELISA, WB and reacts with Mumps orthorubulavirus, Recombinant full length protein - Mumps orthorubulavirus samples. Cited in 1 publication.

別名を表示する

NP, Nucleocapsid protein, Nucleoprotein

1 Images
Western blot - Anti-Mumps nucleoprotein antibody [5E3] (AB9879)
  • WB

Unknown

Western blot - Anti-Mumps nucleoprotein antibody [5E3] (AB9879)

Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 μg/ml. Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 µg/ml.

All lanes:

Western blot - Anti-Mumps nucleoprotein antibody [5E3] (ab9879)

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

5E3

アイソタイプ

IgG2a

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Mumps orthorubulavirus

アプリケーション

WB, I-ELISA

applications

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mumps orthorubulavirus": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "1/1000 - 1/4997", "IELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Mumps orthorubulavirus": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

製品の詳細

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The mumps nucleoprotein sometimes referred to as N protein plays an important role in the replication and transcription of the mumps virus acting as a structural element in the formation of viral ribonucleoprotein (RNP) complexes. The nucleoprotein is a large protein with a molecular mass of approximately 60 kDa and is expressed in cells that are infected by the mumps virus. It facilitates encapsidation of viral RNA and ensures the stability of the viral genome which is essential for virus survival and propagation.
Biological function summary

The mumps nucleoprotein functions as a part of the mumps virus replication complex. It interacts with other viral proteins such as the phosphoprotein (P protein) and the large protein (L protein) to initiate and regulate the replication of viral RNA. These interactions are critical for the stabilization of the entire complex ensuring the efficient production of new viral particles within host cells. The nucleoprotein is primarily located in the cytoplasm of infected cells where it associates with newly synthesized viral RNA strands.

Pathways

The mumps nucleoprotein is involved in the viral replication and transcription pathways specific to the mumps virus. These pathways facilitate viral spread and infection contributing to the virulence of the virus. The nucleoprotein works closely with L and P proteins which serve vital roles in the RNA polymerase complex guiding the synthesis of viral RNA. These interactions are part of the broader negative-strand RNA virus replication pathway shared by other viruses in the Paramyxoviridae family.

The mumps nucleoprotein is directly associated with the mumps disease a contagious viral infection that can lead to severe complications such as encephalitis and orchitis. Antibodies targeting the nucleoprotein can help in diagnosing mumps infections. The nucleoprotein also has implications in mumps-related complications where it interacts with immune system components. Research into this protein helps in understanding the immunological response to the mumps virus potentially informing vaccine development and antiviral therapies.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of medical virology 66:123-30 PubMed11748668

2001

High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology.

Applications

Unspecified application

Species

Unspecified reactive species

Dhanraj Samuel,Kestutis Sasnauskas,Li Jin,Stuart Beard,Aurelija Zvirbliene,Alma Gedvilaite,Bernard Cohen
View all publications

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