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Anti-Mucin 5AC 抗体 [2-11M1] (ab24071)

Submit a review Q&A (5)References (24)
Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (ab24071)

    Key features and details

    • Mouse monoclonal [2-11M1] to Mucin 5AC
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Mucin 5AC antibody [2-11M1]
      Mucin 5AC 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [2-11M1] to Mucin 5AC

    • 由来種

      Mouse

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification) Human.

    • 特記事項


      This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • バッファー

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Protein G purified

    • 一次抗体 備考

      This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.

    • ポリ/モノ

      モノクローナル

    • クローン名

      2-11M1

    • ミエローマ

      Sp2/0

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab24071の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    特記事項
    Flow Cyt
    Use 2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.

    • 組織特異性

      Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.

    • 配列類似性

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 3 TIL (trypsin inhibitory-like) domains.
      Contains 4 VWFC domains.
      Contains 4 VWFD domains.

    • ドメイン

      The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.

    • 翻訳後修飾

      C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis.
      Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Apomucin antibody
        • Apomucin Major Airway Glycoprotein antibody
        • Gastric mucin antibody
        • LeB antibody
        • Lewis B blood group antigen antibody
        • Major airway glycoprotein antibody
        • MUC 5 antibody
        • MUC-5AC antibody
        • MUC5 antibody
        • MUC5A_HUMAN antibody
        • MUC5AC antibody
        • Mucin 5 subtype AC tracheobronchial antibody
        • Mucin 5 Subtypes A And C antibody
        • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
        • Mucin 5, tracheobronchial/gastric antibody
        • Mucin 5AC antibody
        • Mucin 5AC oligomeric mucus/gel forming antibody
        • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
        • Mucin antibody
        • Mucin-5 subtype AC antibody
        • Mucin-5AC antibody
        • TBM antibody
        • tracheobronchial antibody
        • Tracheobronchial Mucin (TBM) antibody
        • Tracheobronchial mucin antibody
        see all

      画像

      • Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (ab24071)
        Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (ab24071)
        Overlay histogram showing A549 cells stained with ab24071 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24071, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      参考文献 (24)

      ab24071 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab24071 は 24 報の論文で使用されています。

      • Wang Z  et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol 19:210-221 (2022). PubMed: 34983944
      • Huang X  et al. MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model. Respir Res 23:11 (2022). PubMed: 35042537
      • Morgan R  et al. A medium composition containing normal resting glucose that supports differentiation of primary human airway cells. Sci Rep 12:1540 (2022). PubMed: 35087167
      • Keith JD  et al. Muc5b Contributes to Mucus Abnormality in Rat Models of Cystic Fibrosis. Front Physiol 13:884166 (2022). PubMed: 35574458
      • Jo WS  et al. Low Dose Rate Radiation Regulates M2-like Macrophages in an Allergic Airway Inflammation Mouse Model. Dose Response 20:15593258221117349 (2022). PubMed: 36003321
      View all Publications for this product

      レビューと Q&A

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      レビューを送る 質問を送る

      1-5 of 5 Abreviews or Q&A

      Question

      Please see below an enquiry we have received from a customer;

      Are you able to tell me the difference between these antibodies (apart from the breadth of use) and do you have access to papers demonstrating immunohisto preferably in cow. Thanks for your help.

      If you wouldn't mind contacting Abcam that would be fantastic. I am wanting to use either of these antibodies for IHC(fr) on bovine lung tissue and would ideally like to know that either works for this application on bovine tissue. AB24071 says it works in bovine but I am unsure whether this applies to WB or IHC or both.

      Read More
      Answer

      Thank you for contacting us.

      While we are unaware of any references showing this, both ab24071 and ab24072 will be guaranteed to react with cow Mucin 5AC in IHC-Fr. These antibodies are very similar and should give similar results, the only difference is that are different clones and may recognize slightly different epitopes. Both epitopes are located on the peptide core of gastric mucin.

      I hope thishelps, please let me know if you have any additional questions!

      Read More

      Question

      Dear Abcam, I am looking at the supernatant from tracheal epithelial cells. The supernatant is not processed. I will have a try with the 1:100 concentration. Any other suggestions would be welcome. Thanks,

      Read More
      Answer

      Thanks for your reply. Are these are human tracheal epithelial cells? In addition to the 1:100 antibody dilution, you should also stain your membrane with Ponceau dye to make sure that the protein transferred successfully. I look forward to hearing from you and I hope these suggestions will produce good results. Please keep me updated and let me know if you have any further questions.

      Read More

      Question

      Dear Abcam, I have loaded 40ug protein. The sample is a supernatant with medium containing serum. I am using laemmli buffer and milk for antibody(1:1000) overnight at 4 degrees. Thanks for the help.

      Read More
      Answer

      My colleague Heather is out of the office but I will be happy to help you further with this. I have a couple of additional questions as well. What kind of cells are you studying? Is the supernatant processed before being loaded into the gel? We would recommend starting with a 1 ug/mL concentration of the antibody, which would be a 1:100 dilution. This should help increase the band strength. I look forward to hearing from you and resolving this promptly. Please let me know if you have any questions.

      Read More

      Question

      We have tested the periodate treatment and there's still no binding. We think that this antibody is not suitable for IHC on bovine tissue, in spite of what it states on your website. Could we get a refund on this antibody?

      Read More
      Answer

      I am very sorry to hear the pretreatment did not resolve the problem. I understand your position and I regret not to be able to give you more specific information, I have arranged a refund on your order. Best of luck with your experiments. I hope this will not deter you from purchasing from Abcam in the future.

      Read More

      Question

      I still haven't heared anything about the MUC5AC antibody. Have you got any information from the lab that tested it on bovine tissue?

      Read More
      Answer

      I was informed today that pretreatment with periodate solution (0.1 - 0.05M) is recommended for 10-20 minutes when detectin mucin in IHC. There are 2 publications which describe the periodate method: Int.J. Cancer 47; 304-310 (1991) J.Immunol.Methods 149: 105-113 (1992) Unfortunately we are unable to download the articles for more details, I'm very sorry I cannot help you more and hope the articles will provide more details. Please do not hesitate to contact me if you need further assistance and I will do my best to help you.

      Read More

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