AB77995

Anti-MUC5B 抗体 [19.4E]

Name: Anti-MUC5B 抗体 [19.4E] (ab77995) | Abcam
Brand: Abcam Limited
SKU: AB77995
Price: 71000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-MUC5B antibody [19.4E]

(2 Reviews)

(30 Publications)

Anti-MUC5B antibody [19.4E] (ab77995) is a mouse monoclonal antibody detecting MUC5B in Western Blot, IHC-P, IHC-Fr, ELISA. Suitable for Human, Monkey, Mouse.

- Over 20 publications
- Trusted since 2009

別名を表示する

MUC5, MUC5B, Mucin-5B, MUC-5B, Cervical mucin, High molecular weight salivary mucin MG1, Sublingual gland mucin

2 Images

Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)

ab77995 staining MUC5B in murine asthma lung tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with formaldehyde, followed by incubation with the primary antibody at 4μg/ml for 16 hours at 4°C. ab77995 was coupled to biotin and Streptavidin 488 (1/250) was used to amplify the signal. Counterstained with DAPI (blue).

This image was generated using the ascites version of the product.

Image courtesy of Dr Francois Daubeuf by Abreview.

CiteAb

Western blot - Anti-MUC5B antibody [19.4E] (AB77995)

Western Blotting using Anti-MUC5B antibody [19.4E], ab77995. Publication image from Liu, Y. et al., 2020, Cell Res, 33159154. Legend direct from paper.

IFN-β and IFN-γ activated the Kyn-AhR pathway for mucin expression.a PAECs or BEAS-2B cells were treated with IFN-β (1 ng/mL) or IFN-γ (10 ng/mL) for 48 h. The expression of IDO1 was determined by western blotting. b BEAS-2B cells were treated with IFN-β (1 ng/mL) or IFN-γ (10 ng/mL) for 48 h. The levels of Kyn from cell lysates or the supernatants were determined by ELISA. c PAECs were isolated from AhR–/– mice were treated with IFN-β (1 ng/mL) or IFN-γ (10 ng/mL) for 24 h. The expression of Mucs 1, 2, 5A, 6, 13, 15 and 16 was determined by real-time PCR. d BEAS-2B cells were treated with Kyn (400 µM) for 24 h. The expression of mucins (MUCs 2, 5A, 6, 7, 13, 15, 16, 17, 18 and 20) was determined by real-time PCR. e Western blot analysis of the expression of mucins 1, 5A, 5B, 13 and 16 from BEAS-2B cells or PAECs treated with Kyn (400 µM) for 48 h. The data represent means ± SD. In b–d, n = 3 biological independent samples. Representative images are from three independent experiments (a and e). *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed Student’s t-test (d) or one-way ANOVA (b and c).

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

19.4E

アイソタイプ

IgG1

キャリアフリー

交差種

Mouse, Human, Monkey

アプリケーション

IHC-P, ELISA, WB, IHC-Fr

applications

免疫原

Synthetic Peptide within Human MUC5B. The exact immunogen used to generate this antibody is proprietary information.

Q9HC84

特異性

Does not react with human Mucin 5A or 5C.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Deglycosylating the tissue is critical to expose epitope.", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Deglycosylating the tissue is critical to expose epitope.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Monkey": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Deglycosylating the tissue is critical to expose epitope.", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-MUC5B antibody [19.4E] (ab77995) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human, Monkey, Mouse samples.

Trusted by the scientific community
Anti-MUC5B [19.4E] (ab77995) was first used in a scientific publication in 2009 and has been cited over 20 times in peer-reviewed journals.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.1% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.

See full target information MUC5B

style

left-column

文献 (30)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869396

2025

Liquiritin Suppresses Intracellular and Secreted MUC5AC and MUC5B in Human Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ryoma Yoshio,Jun Iwashita

ImmunoTargets and therapy 13:789-803 PubMed39712952

2024

Lipopolysaccharide in Bile Promotes the Neutrophil Extracellular Traps-Induced Gallstone Formation by Activating the Gallbladder Immune Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Yu,Ziang Meng,Xuxu Liu,Yi Zheng,Dongbo Xue,Chenjun Hao,Liyi Wang

International journal of chronic obstructive pulmonary disease 19:1635-1647 PubMed39045541

2024

Cigarette Smoke Extract Induces MUC5AC Expression Through the ROS/ IP3R/Ca Pathway in Calu-3 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wu,Guoyue Zhang,Xianzhi Du

International journal of molecular medicine 53: PubMed38240085

2024

NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhou,Qun Zhang,Chenyang Liu,Jiahao Fan,Wen Huang,Nan Li,Mingxia Yang,Hong Wang,Weiping Xie,Hui Kong

Nature cardiovascular research 2:449-466 PubMed39196043

2023

Three-dimensional morphologic and molecular atlases of nasal vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Seon Pyo Hong,Myung Jin Yang,Jung Hyun Bae,Du Ri Choi,Young-Chan Kim,Myeon-Sik Yang,Byungkwan Oh,Kyung Won Kang,Sang-Myeong Lee,Bumseok Kim,Yong-Dae Kim,Ji Hoon Ahn,Gou Young Koh

Frontiers in pharmacology 13:851374 PubMed36188610

2022

Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Iwashita,Hikari Maeda,Momo Ishimura,Jun Murata

iScience 25:104685 PubMed35789750

2022

Immunomodulatory LncRNA on antisense strand of ICAM-1 augments SARS-CoV-2 infection-associated airway mucoinflammatory phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Dinesh Devadoss,Arpan Acharya,Marko Manevski,Dominika Houserova,Michael D Cioffi,Kabita Pandey,Madhavan Nair,Prem Chapagain,Mehdi Mirsaeidi,Glen M Borchert,Siddappa N Byrareddy,Hitendra S Chand

Scientific reports 12:8742 PubMed35610327

2022

The normal and fibrotic mouse lung classified by spatial proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Ciccimarra,Maddalena M Bolognesi,Matteo Zoboli,Giorgio Cattoretti,Franco F Stellari,Francesca Ravanetti

Nature protocols 17:1658-1690 PubMed35546639

2022

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.

Applications

Unspecified application

Species

Unspecified reactive species

Rajendra Kumar Gurumurthy,Stefanie Koster,Naveen Kumar,Thomas F Meyer,Cindrilla Chumduri

Respiratory research 23:11 PubMed35042537

2022

MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Huang,Weijie Guan,Bin Xiang,Wei Wang,Yanqing Xie,Jinping Zheng

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MUC5B 抗体 [19.4E]

Anti-MUC5B antibody [19.4E]

Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)

Western blot - Anti-MUC5B antibody [19.4E] (AB77995)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (30)

Abcam product promise