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Anti-MUC4 抗体 [5B12] (ab60720)

Reviews (2)Q&A (3)References (23)
Western blot - Anti-MUC4 antibody [5B12] (ab60720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [5B12] (ab60720)
  • Flow Cytometry - Anti-MUC4 antibody [5B12] (ab60720)

Key features and details

  • Mouse monoclonal [5B12] to MUC4
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-MUC4 antibody [5B12]
    MUC4 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5B12] to MUC4

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment with tag: GVSLFPYGAD AGDLEFVRRT VDFTSPLFKP ATGFPLGSSL RDSLYFTDNG QIIFPESDYQ IFSYPNPLPT GFTGRDPVAL VAPFWDDADF STGRGTTFYQ EYETFYGEHS , corresponding to amino acids 79-189 of Human MUC4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: HeLa cell lysate. IHC-P: Human stomach tissue. Flow Cyt: A549 cells.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 12 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab60720の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 235 kDa).
IHC-P (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 235 kDa).
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.

  • 組織特異性

    Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.

  • 配列類似性

    Contains 1 AMOP domain.
    Contains 2 EGF-like domains.
    Contains 1 NIDO domain.
    Contains 1 VWFD domain.

  • 発生段階

    Expressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation.

  • 翻訳後修飾

    Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
    mucrnin-4 alpha chain is highly O-glycosylated.
    mucin-4 beta chain is predominantly N-glycosylated.

  • 細胞内局在

    Secreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Ascites sialoglycoprotein 1 antibody
      • Ascites sialoglycoprotein 2 antibody
      • Ascites sialoglycoprotein antibody
      • ASGP antibody
      • ASGP-1 antibody
      • ASGP-2 antibody
      • HSA276359 antibody
      • MUC 4 antibody
      • MUC-4 antibody
      • Muc4 antibody
      • MUC4_HUMAN antibody
      • Mucin 4 antibody
      • Mucin 4 cell surface associated antibody
      • Mucin 4 tracheobronchial antibody
      • Mucin-4 beta chain antibody
      • Pancreatic adenocarcinoma mucin antibody
      • Testis mucin antibody
      • Tracheobronchial mucin antibody
      • Tracheobronchial mucin Fragment antibody
      see all

    画像

    • Western blot - Anti-MUC4 antibody [5B12] (ab60720)
      Western blot - Anti-MUC4 antibody [5B12] (ab60720)
      Anti-MUC4 antibody [5B12] (ab60720) at 1 µg/ml + HeLa cell lysate at 25 µg

      Predicted band size: 235 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?
      Additional bands at: 52 kDa, 65 kDa. We are unsure as to the identity of these extra bands.



      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [5B12] (ab60720)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [5B12] (ab60720)

      MUC4 antibody (ab60720) used in immunohistochemistry at 3ug/mL on formalin fixed and paraffin embedded human stomach.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-MUC4 antibody [5B12] (ab60720)
      Flow Cytometry - Anti-MUC4 antibody [5B12] (ab60720)

      Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

      This image was generated using the ascites version of the product.

    参考文献 (23)

    ab60720 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab60720 は 23 報の論文で使用されています。

    • Lungova V  et al. Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling. Dis Model Mech 15:N/A (2022). PubMed: 35770504
    • He N  et al. Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection. Commun Biol 5:660 (2022). PubMed: 35787656
    • Lu D  et al. The Mechanism of Lipopolysaccharide's Effect on Secretion of Endometrial Mucins in Female Mice during Pregnancy. Int J Mol Sci 23:N/A (2022). PubMed: 36077364
    • Collin J  et al. Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface. Ocul Surf 19:190-200 (2021). PubMed: 32502616
    • Cotoraci CA  et al. The morphological changes of the colonic goblet cells and mucin profile in oncohematological patients under Epirubicin-based chemotherapy. Rom J Morphol Embryol 61:1121-1128 (2020). PubMed: 34171061
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC4 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (oviductal tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
    Permeabilization
    No
    Specification
    oviductal tissue
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 27 2016

    Western blot abreview for Anti-MUC4 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Serum (mouse serum)
    Loading amount
    30 µg
    Specification
    mouse serum
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 May 25 2012

    Question

    ab60720: immunogen available for sale? or full length protein?

    Read More
    Answer

    Thank you for your patience.
    The immunogen has now been added to our catalog as ab130768.
    https://www.abcam.com/Recombinant-Human-MUC4-protein-ab130768.html
    This is the immunogen (protein fragment) that was used to raise antibody ab60720.
    If you would order the item today, you would receive it in 2-3 weeks.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    ab60720: immunogen available for sale? or full length protein?

    Read More
    Answer

    Thank you for contacting us.
    The lab let me know that the immunogen would be available for sale. I do not have yet any information about catalog number, amount and price, but will let you know as soon as I do.
    I hope this is good news for you. Please do not hesitate to contact us if you need any more advice or information in the meantime.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear abcam technical support team: This customer would like to order
    ab60720 (Anti-MUC4 antibody),
    ab86704 (Anti-Villin antibody [M245]),
    ab68715 (Anti-SETBP1 antibody),
    ab86177 (Anti-CTRB1 antibody),
    ab21156 (Anti-Pancreatic Amylase antibody) and
    ab65109 (Anti-MUC13 antibody),
    he would likes to order these antibodies and test in wb with mouse sample, would you please help this customer to create a discount code, the information of this customer as follow:
    Could you please help this customer to solve these problems? Thanks for your kindly help. Best regards

    Read More
    Answer

    Thank you for your inquiry.

    Unfortunately, I am unable to issue discount code for the following antibodies:

    ab60720, ab86704 and ab65109.

    The immunogens for the MUC antibodies do not show a high enough homology (70% and below)to mouse to expect any cross-reactivity. Unfortunately, this is also true for the other antibodies we offer against these targets too.

    ab86704 is already tested and guaranteed for mouse and WB. Please reassure your customer that the Abpromise guarantee will apply to this antibody in their experiment.

    DISCOUNT CODEs:

    ab68715: xxxxx


    ab86177: xxxxx


    ab21156: xxxxx

    Expiration date:17. May 2012

    I am very pleased to hear your customer would like to accept our offer and test ab68715 and ab86177 and ab21156 in mouse.

    The codes will give your customer 1 freeprimary antibody eachbefore the expiration date.

    To redeem this offer, please submit an Abreview eachformouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

    1) Call to place your order and mention the code to our customer service department;

    2) Include the code in your fax order;

    3) Place your order on the web and enter the promotional code.

    Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com