Anti-MUC2 抗体 [996/1] (ab11197)

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

Should you have any further questions, please do not hesitate to contact us.

Thank you for contacting us.

According to our Technical FAQs, we would like to suggest to use 1 ug/ml as a starting concentration for this antibody.



Regarding the gel we would like suggest to use a gel between 5% and 7.5%. Please take care with the handling as they are very delicate and easy to rip.

In addition, here's a protocol for the transfer/blotting of this heavy protein:

1. Don't use PVDF membrane, nitrocellulose membrane works much better.
2. Use Tris-glycine based buffers.
3. Transfer should be longer and lower temperature (overnight at 4C and at 30V).
4. Use 2 pieces of Wattman for each part of the "sandwich".
5. Add some more SDS to the system than for normal standard WB, add 2 ml of 10% SDS to 2 l of transfer buffer which is Tris based.

Transfer Buffer:
39 mM Glycine
48 mM Tris Base
0.037% SDS (electrophoresis grade)
20% Methanol



In addition, a wet transfer may be more sufficient than a semi-dry.



Finally, please see the reference we have on our website under"Specific References":

Guilmeau S et al. Heterogeneity of Jagged1 expression in human and mouse intestinal tumors: implications for targeting Notch signaling. Oncogene : (2009). WB; Mouse.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting Abcam.
We have not specifically tested this antibody on immature and mature mucin. However, I have done some research. There's a paper which looks at O-glycosylation of MUC2. Specifically, this paper notices aberrant glycosylation of MUC-2 in diseased tissue. Because this antibody recognizes both malignant and normal mucosa and the O-glycosylation differs depending on the disease state, it is unlikely that the glycosylation state of the protein has an effect on antibody recognition.
I hope this helps, but please let me know if you have any follow up questions.