AB45167

Anti-MUC1 抗体 [EP1024Y]

Name: Anti-MUC1 抗体 [EP1024Y] (ab45167) | Abcam
Brand: Abcam Limited
SKU: AB45167
Price: 29000 JPY
Availability: InStock
Rating: 3 (4 reviews)

Anti-MUC1 antibody [EP1024Y]

RabMAb
Recombinant
KO Validated
20ul selling size
詳細を見る

(4 Reviews)

(44 Publications)

Anti-MUC1 antibody [EP1024Y] (ab45167) is a rabbit monoclonal antibody detecting MUC1 in Western Blot, Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
- Trusted since 2006

別名を表示する

CD227, PUM, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin, CA 15-3, KL-6, PEM, EMA

7 Images

Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (AB45167)

Flow Cyt

Unknown

Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (AB45167)

Flow Cytometry analysis of A549 (human lung carcinoma cell line) cells labeling MUC1 with purified ab45167 at 1/20 dilution (10 ug/ml). Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution. Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabeled control, cells without incubation with primary antibody and secondary antibody.

Flow Cyt

Unknown

Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (AB45167)

Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) cells labelling MUC1 with unpurified ab45167 (pink) at a dilution of 1/150. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as the isotype control (green).

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Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] (AB45167)

ab45167 (purified) at 1/20 dilution (2ug) immunoprecipitating MUC1 in Human fetal lung lysate.

Lane 1 (input) : Human fetal lung lysate 10ug

Lane 2 (+) : ab45167 + Human fetal lung lysate 10ug

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab45167 in Human fetal lung lysate

For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] (ab45167)

Predicted band size: 122 kDa

Observed band size: 27 kDa

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Lab

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

Lanes 1- 2 : Merged signal (red and green). Green - ab45167 observed at 24 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab45167 was shown to react with MUC1 in Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab255412 (knockout cell lysate ab263764) was used. Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) and MUC1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab45167 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Lane 2:

T-47D (Human ductal breast epithelial tumor cell line) cell lysate at 20 µg

Predicted band size: 122 kDa

Observed band size: 24 kDa

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Lab

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/1000 dilution

Lane 1:

Human colon cancer lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 122 kDa

Observed band size: 27 kDa

false

Lab

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/2000 dilution

All lanes:

T47D cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 122 kDa

Observed band size: 27 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/5000 dilution

All lanes:

Mouse liver lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 122 kDa

Observed band size: 27 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1024Y

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

Flow Cyt, WB, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Based on the immunogen sequence, the antibody recognises several isoforms of MUC1 (Uniprot ID P15941). They are Isoform Y (28 kDa), Isoform Y-LSP (28 kDa), Isoform S2 (17 kDa) and Isoform J13 (28 kDa).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "For unpurified use at 1/50.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "MUC1 isoform 7.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "MUC1 isoform 7.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "MUC1 isoform 7.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-MUC1 antibody [EP1024Y] (ab45167) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-MUC1 [EP1024Y] (ab45167) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-MUC1 antibody [EP1024Y] (ab45167) has been confirmed by Western blot testing in Mucin-1 Knockout HeLa cells.

Other related products
We have a range of other formats of antibody clone [EP1024Y] also available for your convenience: ab45167, PE - ab213337, Carrier free - ab218998, Carrier free - ab256588, APC - ab317951, Alexa Fluor® 488 - ab317992, Alexa Fluor® 594 - ab318034, Alexa Fluor® 555 - ab318076, Alexa Fluor® 647 - ab318117

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

See full target information Mucin-1

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文献 (44)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6032 PubMed40595652

2025

EZH2 loss promotes gastric squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengsha Zhang,Ailing Zhong,Hongyu Liu,Lei Zhao,Yingjie Wang,Zhenghao Lu,Lanxin Zhang,Xiangyu Pan,Zuoyu Liang,Limin Gao,Xuelan Chen,Jian Wang,Siqi Dai,Xudong Wan,Manli Wang,Jingyao Chen,Jiajia Du,Shuang Chen,Tingfa Peng,Jingyang Cai,Liuxiang Chen,Yang Yang,Chaoxin Xiao,Menglin Yao,Jing Xu,Runkuan Qin,Chan Yang,Baohong Wu,Qi Zhang,Shengzhuo Liu,Chengjian Zhao,Hongxin Deng,Yuan Wang,Lu Chen,Jiankun Hu,Bing Hu,Shengyong Yang,Feifei Na,Linjie Guo,Yu Liu,Chong Chen

International journal of molecular sciences 26: PubMed40362368

2025

Effects of Acrylamide on Mouse Implantation and Decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yuan Yang,Hui-Na Luo,Zai-Mei Wang,Dan-Dan Jin,Zeng-Ming Yang

International journal of molecular sciences 26: PubMed40362361

2025

Effect of Diethylstilbestrol on Implantation and Decidualization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Ran,Si-Ting Chen,Meng-Yuan Li,Dan-Dan Jin,Zeng-Ming Yang

Cell death & disease 15:605 PubMed39168965

2024

Neoplastic ICAM-1 protects lung carcinoma from apoptosis through ligation of fibrinogen.

Applications

Unspecified application

Species

Unspecified reactive species

ShiHui Wang,JunLei Wang,Cui Liu,Lei Yang,XuanQian Tan,ShiYang Chen,Yun Xue,HongBin Ji,GaoXiang Ge,JianFeng Chen

Aging cell 23:e14303 PubMed39113346

2024

The activation of cGAS-STING pathway causes abnormal uterine receptivity in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Ting Chen,Wen-Wen Shi,Feng Ran,Cheng-Kan Liu,Hui-Na Luo,Li-Juan Wu,Ying Wu,Tong-Tong Zhang,Zeng-Ming Yang

Heliyon 10:e33938 PubMed39071710

2024

MiR-30c suppresses the proliferation, metastasis and polarity reversal of tumor cell clusters by targeting MTDH in invasive micropapillary carcinoma of the breast.

Applications

Unspecified application

Species

Unspecified reactive species

Yunwei Han,Weidong Li,Renyong Zhi,Gui Ma,Ang Gao,Kailiang Wu,Hui Sun,Dan Zhao,Yiling Yang,Fangfang Liu,Feng Gu,Xiaojing Guo,Jintang Dong,Shuai Li,Li Fu

Langmuir : the ACS journal of surfaces and colloids 40:9926-9933 PubMed38683632

2024

Bioinspired Lubricity from Surface Gel Layers.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Al Kindi,Nemea S Courelli,Kevin Ogbonna,Juan Manuel Urueña,Allison L Chau,Angela A Pitenis

BMC infectious diseases 23:845 PubMed38036963

2023

Role of the cAMP-PKA-NF-κB pathway in Mucin1 over-expression in A549 cells during Respiratory syncytial virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yingkang Jin,Dongwei Zhang,Kuimiao Deng,Peiqiong Wu,Diyuan Yang,Zhiwei Xie,Wenjun Qiu,Guangyuan Yu

Annals of translational medicine 10:1361 PubMed36660692

2023

Mucin 1 downregulation decreases the anti-tumor effects of melanoma vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Shi,Rui Xue,Hui Xu,Feng Mei,Xueyang Bao,Jun Dou,Fengshu Zhao

Cancer immunology research 11:320-338 PubMed36603133

2023

CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Lao,Wenfeng Zeng,Penghan Huang,Huiping Chen,Zishuo Jia,Pei Wang,Di Huang,Jianing Chen,Yan Nie,Linbin Yang,Wei Wu,Jiang Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MUC1 抗体 [EP1024Y]

Anti-MUC1 antibody [EP1024Y]

Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (AB45167)

Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (AB45167)

Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] (AB45167)

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

Western blot - Anti-MUC1 antibody [EP1024Y] (AB45167)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (44)

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