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リコンビナントRabMAb

Anti-Mu Opioid Receptor 抗体 [EPR26293-61] (BSA and Azide free) (ab302513)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
  • Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26293-61] to Mu Opioid Receptor - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free)
    Mu Opioid Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26293-61] to Mu Opioid Receptor - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC/IF,IHC-Fr,IP or WB

  • 種交差性

    交差種: Rat
    非交差種: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Rat spinal cord and cerebrum FFPE tissue sections; HEK-293T cells transfected with a rat OPRM1 expression vector containing a myc-His tag cell pellet.

  • 特記事項

    ab302513 is the carrier-free version of ab302512.

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302513の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF,IHC-Fr,IP or WB.

ターゲット情報

  • 機能

    Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • hMOP antibody
      • LMOR antibody
      • M-OR-1 antibody
      • MOP antibody
      • MOR antibody
      • MOR-1 antibody
      • MOR1 antibody
      • Mu opiate receptor antibody
      • Mu opioid receptor antibody
      • Mu-type opioid receptor antibody
      • Opioid receptor mu 1 antibody
      • OPRM antibody
      • OPRM_HUMAN antibody
      • OPRM1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)

      This data was developed using ab302512, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded HEK-293T cells labeling Mu Opioid Receptor. The section was incubated with ab302512 for 30 mins at room temperature at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Cytoplasmic staining on A: HEK-293T cells transfected with a rat OPRM1 expression vector containing a myc-His tag cell pellet, and no staining on B: 293T transfected with an empty vector cell pellet. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)

      This data was developed using ab302512, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Mu Opioid Receptor with ab302512 at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Negative control: no staining on rat liver. The section was incubated with ab302512 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)

      This data was developed using ab302512, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Mu Opioid Receptor with ab302512 at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on rat cerebrum. The section was incubated with ab302512 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)

      This data was developed using ab302512, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling Mu Opioid Receptor with ab302512 at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on rat spinal cord. The section was incubated with ab302512 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)
      Anti-Mu Opioid Receptor antibody [EPR26293-61] (BSA and Azide free) (AB302513)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302513 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302513 は論文での使用が確認できていません。

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