Anti-MTTP/MTP 抗体 (ab63467)
Key features and details
- Rabbit polyclonal to MTTP/MTP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTTP/MTP antibody
MTTP/MTP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MTTP/MTP -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in Human Liver Tissue Lysate. ICC/IF: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63467の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
ターゲット情報
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機能
Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. -
組織特異性
Liver and small intestine. Also found in ovary, testis and kidney. -
関連疾患
Abetalipoproteinemia -
配列類似性
Contains 1 vitellogenin domain. -
細胞内局在
Endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 4547 Human
- Omim: 590075 Human
- SwissProt: P55156 Cow
- SwissProt: P55157 Human
- Unigene: 195799 Human
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別名
- ABL antibody
- Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
- Microsomal triglyceride transfer protein 88kD antibody
see all
画像
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Western blot - Anti-MTTP/MTP antibody (ab63467)Anti-MTTP/MTP antibody (ab63467) at 1 µg/ml + Liver (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-MTTP/MTP antibody (ab63467)ab63467 staining MTTP/MTP in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab63467 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab63467 は 7 報の論文で使用されています。
- Hendriks D et al. Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis. Nat Biotechnol 41:1567-1581 (2023). PubMed: 36823355
- Yadav PK et al. Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cells. J Biol Chem 298:101813 (2022). PubMed: 35278429
- Anaganti N et al. Generation of hepatoma cell lines deficient in microsomal triglyceride transfer protein. J Lipid Res 63:100257 (2022). PubMed: 35931202
- Zheng YY et al. Synthesis of Novel MicroRNA-30c Analogs to Reduce Apolipoprotein B Secretion in Human Hepatoma Cells. Bio Protoc 12:N/A (2022). PubMed: 36618093
- Hu L et al. Avasimibe: A novel hepatitis C virus inhibitor that targets the assembly of infectious viral particles. Antiviral Res 148:5-14 (2017). PubMed: 29074218
- Bai J et al. Sodium sulfite promotes the assembly and secretion of very low-density lipoprotein in HL-7702 hepatocytes. Toxicol Rep 3:98-104 (2016). PubMed: 28959527
- Yamamoto Y et al. Rapid alternative absorption of dietary long-chain fatty acids with upregulation of intestinal glycosylated CD36 in liver cirrhosis. Am J Clin Nutr 96:90-101 (2012). Human . PubMed: 22648712