Anti-MTR 抗体 (ab66039)
Key features and details
- Rabbit polyclonal to MTR
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTR antibody
MTR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MTR -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Horse, Pig, Caenorhabditis elegans, Chimpanzee
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human MTR.
Immunogen の所有権に関して (Peptide available as ab69085.) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: TE 671 Whole Cell, Mouse Liver Tissue, Rat Thymus Tissue, Mouse Pancreas Tissue; Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Staurosporine Treated (24hr, 500nM), HeLa Whole Cell - Bleomycin Treated (20U/ml), HeLa Whole Cell, Mouse Kidney Tissue, Rat Liver Tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab66039の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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関連性
MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 4548 Human
- Entrez Gene: 238505 Mouse
- Entrez Gene: 81522 Rat
- NCBI: 4557765 Human
- SwissProt: Q99707 Human
- SwissProt: A6H5Y3 Mouse
- SwissProt: Q9Z2Q4 Rat
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別名
- 5-methyltetrahydrofolate homocysteine methyltransferase antibody
- 5-methyltetrahydrofolate-homocysteine methyltransferase antibody
- cblG antibody
see all
画像
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Western blot - Anti-MTR antibody (ab66039)All lanes : Anti-MTR antibody (ab66039) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : Pancreas (Mouse) Tissue Lysate
Lane 5 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 6 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 7 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 : Kidney (Mouse) Tissue Lysate
Lane 10 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141 kDa
Additional bands at: 117 kDa, 35 kDa, 50 kDa, 55 kDa, 65 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-MTR antibody (ab66039)ICC/IF image of ab66039 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66039, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR antibody (ab66039)IHC image of ab66039 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66039, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab66039 は 9 報の論文で使用されています。
- Li T et al. Increased homocysteine regulated by androgen activates autophagy by suppressing the mammalian target of rapamycin pathway in the granulosa cells of polycystic ovary syndrome mice. Bioengineered 13:10875-10888 (2022). PubMed: 35485387
- Kim M et al. Duodenal-jejunal bypass maintains hepatic S-adenosylmethionine/S-homocysteine ratio in diet-induced obese rats. Surg Obes Relat Dis 17:1359-1368 (2021). PubMed: 33753007
- Sullivan MR et al. Methionine synthase is essential for cancer cell proliferation in physiological folate environments. Nat Metab 3:1500-1511 (2021). PubMed: 34799701
- Lei L et al. Attenuated expression of MTR in both prenatally androgenized mice and women with the hyperandrogenic phenotype of PCOS. PLoS One 12:e0187427 (2017). PubMed: 29232372
- Bito T et al. A dodecylamine derivative of cyanocobalamin potently inhibits the activities of cobalamin-dependent methylmalonyl-CoA mutase and methionine synthase of Caenorhabditis elegans. FEBS Open Bio 4:722-9 (2014). WB ; Caenorhabditis elegans . PubMed: 25161880
- Pérez-Sepúlveda A et al. Levels of key enzymes of methionine-homocysteine metabolism in preeclampsia. Biomed Res Int 2013:731962 (2013). WB ; Human . PubMed: 24024209
- Cherukad J et al. Spatial and temporal expression of folate-related transporters and metabolic enzymes during mouse placental development. Placenta 33:440-8 (2012). PubMed: 22365888
- Sáez-Ayala M et al. Melanoma coordinates general and cell-specific mechanisms to promote methotrexate resistance. Exp Cell Res 318:1146-59 (2012). PubMed: 22484375
- Murakami M et al. Improving drug potency and efficacy by nanocarrier-mediated subcellular targeting. Sci Transl Med 3:64ra2 (2011). PubMed: 21209412