Anti-mTOR (phospho S2448) 抗体 [EPR426(2)]
Anti-mTOR (phospho S2448) antibody [EPR426(2)]
- BOND RX™ Validated
- RabMAb
- Recombinant
- Lab Essentials
- 20ul selling size
- 詳細を見る
4
(6 Reviews)
|
(519 Publications)
Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) is a rabbit monoclonal antibody detecting mTOR in Western Blot, IHC-P, IHC-Fr, Dot Blot. Suitable for Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 510 publications
別名を表示する
FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, mTOR
- WB
Lab
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
All lanes:
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/5000 dilution
Lane 1:
untreated HEK293 cell lysate at 10 µg
Lane 2:
HEK293 cell lysate treated with alkaline phosphatase at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
false
Exposure time: 3min
- WB
Lab
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
All lanes:
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution
Lane 1:
untreated HEK293 cell lysate at 10 µg
Lane 2:
HEK293 cell lysate treated with alkaline phosphatase at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
false
Exposure time: 3min
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
Immunohistochemical cytoplasmic and nuclear staining of paraffin embedded human endometrium carcinoma with purified ab109268 at a working dilution of 1 in 100. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab109268 at a dilution of 1/50. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
- IHC-Fr
Lab
Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
IHC image of mTOR (phospho S2448) staining in a section of frozen normal human heart performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab109268, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
- WB
Lab
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
All lanes:
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution
Lane 1:
untreated HeLa cell lysate at 10 µg
Lane 2:
HeLa cell lysate treated with insulin at 10 µg
Lane 3:
HeLa cell lystae treated with insulin, and the membrane treated with alkaline phosphatase at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
false
Exposure time: 1min
- WB
Lab
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
All lanes:
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution
Lane 1:
HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg
Lane 2:
HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate at 10 µg
Lane 3:
HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
false
- WB
Lab
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
All lanes:
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution
Lane 1:
untreated NIH/3T3 cell lysate at 10 µg
Lane 2:
NIH/3T3 cell lysate treated with insulin at 10 µg
Lane 3:
NIH/3T3 cell lysate treated with insulin, then the membrane treated wth alkaline phosphatase at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDa
false
Exposure time: 2min
- Dot
Lab
Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)
Dot blot analysis of mTOR (phospho S2448) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2448) phospho peptide with purified ab109268 at a dilution of 1/1000 (0.073ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.
Blocking buffer : 5% NFDM/TBST
Diluting buffer : 5% NFDM /TBST
関連する標識済み抗体及び組成の異なる製品 (1)
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Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free
Reactivity data
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製品の詳細
What is this antibody validated in?
Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Dot Blot in Human, Mouse samples.
What is the molecular weight of mTOR?
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) specifically detects a band for mTOR (UniProt: P42345) at a molecular weight of 289kDa.
Trusted by the scientific community
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) was first used in a scientific publication in 2011 and has been cited over 510 times in peer-reviewed journals.
Reviewed by scientists
Anti-mTOR (phospho S2448) [EPR426(2)] (ab109268) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR426(2)] also available for your convenience: ab109268, Carrier free - ab177734
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MTOR integrates signals from nutrients growth factors and cellular energy status to maintain cellular homeostasis. It forms part of two distinct complexes mTORC1 and mTORC2 which differ in their component proteins and downstream effects. mTORC1 primarily responds to amino acids and regulates protein synthesis through phosphorylation of key substrates like S6K1. On the other hand mTORC2 is important for maintaining cytoskeletal integrity and cell survival highlighting the protein's importance in diverse cellular processes.
Pathways
MTOR plays a pivotal role in the PI3K/AKT/mTOR pathway which governs cell growth proliferation and survival. It also has implications in the regulation of the AMPK pathway which senses cellular energy levels. Through these pathways mTOR interacts with proteins such as AKT and TSC2. The phospho-mTOR specifically the S2448 phospho-mTOR serves as an important functional marker in these signaling cascades linking extracellular signals to downstream cellular responses.
製品プロトコール
- Visit the General protocols
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ターゲットの情報
文献 (519)
Recent publications for all applications. Explore the full list and refine your search
MedComm 4:e433 PubMed38053815
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Pharmacology 109:34-42 PubMed38011839
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Cell death & disease 14:774 PubMed38008826
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Cell death discovery 9:425 PubMed38007535
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Journal of diabetes research 2023:4193309 PubMed38033740
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International journal of molecular sciences 24: PubMed38003735
2023
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American journal of cancer research 13:5656-5666 PubMed38058816
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Polish journal of pathology : official journal of the Polish Society of Pathologists 74:182-193 PubMed37955537
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Immunity, inflammation and disease 11:e1061 PubMed37904714
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Translational neuroscience 14:20220314 PubMed37854583
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