AB1093

Anti-mTOR (phospho S2448) 抗体

Anti-mTOR (phospho S2448) antibody

Lab Essentials
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(23 Publications)

Rabbit Polyclonal MTOR phospho S2448 antibody. Suitable for WB, IHC-P, ELISA and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human MTOR phospho S2448 aa 2400-2500.

別名を表示する

FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, mTOR

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (AB1093)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (AB1093)

Formalin-fixed, paraffin-embedded human kidney tissue, (proximal convulted tubules), labeling mTOR using ab1093 at 5 μg/ml (red). Counterstain is hematoxylin.

Supplier Data

Western blot - Anti-mTOR (phospho S2448) antibody (AB1093)

All lanes:

Western blot - Anti-mTOR (phospho S2448) antibody (ab1093) at 1/1000 dilution

Lane 1:

24hr serum starved 293T whole cell lysate at 20 µg

Lane 2:

24hr serum starved 293T whole cell lysate, treated with IGF-1 (100 ng/ml) for 20 mins prior to harvest at 20 µg

Secondary

All lanes:

HRP Goat-anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 289 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB, ELISA

applications

免疫原

Synthetic Peptide within Human MTOR phospho S2448 aa 2400-2500. The exact immunogen used to generate this antibody is proprietary information.

P42345

特異性

Reactivity occurs with phosphorylated mTOR from human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with mouse and rat is suggested based on protein sequence homologies.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/100000", "ELISA-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The mammalian target of rapamycin commonly known as mTOR is a serine/threonine kinase known for its role in cellular growth and metabolism. It has a molecular weight of approximately 289 kDa. mTOR is expressed in various tissues throughout the body including muscle adipose tissue and the brain. The protein functions as a central regulator of cell proliferation protein synthesis and nutrient signaling. Often researchers utilize mTOR ELISA or mTOR western blot (mTOR WB) methods and mTOR antibodies to study its expression and activity in various biological contexts.

Biological function summary

MTOR integrates signals from nutrients growth factors and cellular energy status to maintain cellular homeostasis. It forms part of two distinct complexes mTORC1 and mTORC2 which differ in their component proteins and downstream effects. mTORC1 primarily responds to amino acids and regulates protein synthesis through phosphorylation of key substrates like S6K1. On the other hand mTORC2 is important for maintaining cytoskeletal integrity and cell survival highlighting the protein's importance in diverse cellular processes.

Pathways

MTOR plays a pivotal role in the PI3K/AKT/mTOR pathway which governs cell growth proliferation and survival. It also has implications in the regulation of the AMPK pathway which senses cellular energy levels. Through these pathways mTOR interacts with proteins such as AKT and TSC2. The phospho-mTOR specifically the S2448 phospho-mTOR serves as an important functional marker in these signaling cascades linking extracellular signals to downstream cellular responses.

MTOR has connections to cancer and neurodegenerative diseases. Its dysregulation often leads to uncontrolled cellular proliferation a hallmark of many cancers. Conditions such as tuberous sclerosis can occur due to mutations in proteins like TSC1 and TSC2 that regulate mTOR activity. In Alzheimer's disease mTOR's role in autophagy and protein synthesis becomes significant as imbalance may contribute to disease progression. Understanding these connections highlights the potential of targeting mTOR pathways therapeutically.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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ターゲットの情報

The protein expressed by the MTOR gene is a serine/threonine protein kinase that serves as a central regulator of cellular metabolism, growth, and survival in response to various signals, such as hormones and nutrients. MTOR operates within two distinct signaling complexes, mTORC1 and mTORC2. mTORC1 is activated to upregulate protein synthesis by phosphorylating regulators of mRNA translation and ribosome synthesis, and phosphorylates and activates proteins like RPS6KB1 and RPS6KB2 to promote protein synthesis. It controls MiT/TFE factors TFEB and TFE3 by mediating their retention and inactivation under nutrient-rich conditions, and it inhibits autophagy by phosphorylating DAP and RUBCNL/Pacer. Additionally, mTORC1 engages in feedback control on growth factor signaling and may influence microtubules through CLIP1 phosphorylation. The mTORC2 complex may regulate cellular processes, including survival and cytoskeletal organization, by phosphorylating AKT1 and regulating the actin cytoskeleton via PRKCA, PXN, and Rho-type guanine nucleotide exchange factors. It also regulates the phosphorylation of SGK1. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MTOR phospho S2448

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文献 (23)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica Polonica 69:417-422 PubMed35696596

2022

USP18 contributes to the proliferation and migration of ovarian cancer cells by regulating the AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meiqin Liu,Shile Gao,Xingjun Xu,Lijie Zhang,Bin Xu,Donghui Lu

Allergologia et immunopathologia 50:96-103 PubMed35257552

2022

Ubiquitin-specific protease 8 inhibits lipopolysaccharide-triggered pyroptosis of human bronchial epithelial cells by regulating PI3K/AKT and NF-κB pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Liu,Liting Huan,Yu Zhang,Wei Wei,Zhihai Chen,Di Xu,Xiufeng Huang,Yaoxi Tan,Hongxing Li

General physiology and biophysics 40:245-252 PubMed34100380

2021

Tripartite motif containing 28 (TRIM28) promotes the growth and migration of endometrial carcinoma cells by regulating the AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Chen,Haiyan Cheng,Heming Long

International journal of medical sciences 18:2814-2827 PubMed34220309

2021

Abnormal proinflammatory and stressor environmental with increased the regulatory cellular IGF-1/PAPP-A/STC and Wnt-1/β-Catenin canonical pathway in placenta of women with Chronic venous Disease during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Oscar Fraile-Martínez,Miguel A Saez,Miguel A Álvarez-Mon,Ana M Gómez-Lahoz,Coral Bravo,Juan A De León Luis,Felipe Sainz,Santiago Coca,Ángel Asúnsolo,Jorge Monserrat,Luis G Guijarro,Melchor Álvarez-Mon,Julia Bujan,Natalio García-Honduvilla

Cardiovascular research 117:780-791 PubMed32077934

2020

Identification of an amino-terminus determinant critical for ryanodine receptor/Ca2+ release channel function.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Seidel,Camille Rabesahala de Meritens,Louisa Johnson,Dimitris Parthimos,Mark Bannister,Nia Lowri Thomas,Esizaze Ozekhome-Mike,Francis Anthony Lai,Spyros Zissimopoulos

Cardiovascular diagnosis and therapy 9:535-544 PubMed32038943

2020

MiR-216a accelerates proliferation and fibrogenesis via targeting PTEN and SMAD7 in human cardiac fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jinsong Tao,Jingyi Wang,Chunyu Li,Weiwei Wang,Hao Yu,Jinhui Liu,Xiangqing Kong,Yan Chen

Oncology reports 42:2169-2178 PubMed31432154

2019

EMX2 is epigenetically silenced and suppresses epithelial‑mesenchymal transition in human esophageal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Joy Jin,Yong Zhou,Ziqiang Tian,Biao He,Yueyan Huang,Feng Ding

Frontiers in neuroscience 13:769 PubMed31440123

2019

Therapeutic Effect of Modulating TREM-1 via Anti-inflammation and Autophagy in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Wei Feng,Nan-Fu Chen,Chun-Sung Sung,Hsiao-Mei Kuo,San-Nan Yang,Chien-Liang Chen,Han-Chun Hung,Bing-Hung Chen,Zhi-Hong Wen,Wu-Fu Chen

Scientific reports 9:10338 PubMed31316146

2019

Chemopreventive Effect of Phytosomal Curcumin on Hepatitis B Virus-Related Hepatocellular Carcinoma in A Transgenic Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Chiao-Fang Teng,Chun-Hui Yu,Hong-Yi Chang,Wen-Chuan Hsieh,Tzu-Hua Wu,Jia-Hui Lin,Han-Chieh Wu,Long-Bin Jeng,Ih-Jen Su

Molecular medicine reports 20:1187-1195 PubMed31173235

2019

Alisol B 23‑acetate inhibits the viability and induces apoptosis of non‑small cell lung cancer cells via PI3K/AKT/mTOR signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Xi-Chao Xia,Liu-Yang Meng,Yu Wang,Yue-Mei Li

View all publications

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