Anti-MTHFD1L 抗体 (ab116615)
Key features and details
- Goat polyclonal to MTHFD1L
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTHFD1L antibody
MTHFD1L 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MTHFD1L -
由来種
Goat -
特異性
Based on homology with the immunogen sequence, we believe this antibody will also react with MTHFD1 -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human MTHFD1L aa 550-650 (internal sequence) (Cysteine residue).
Database link: NP_056255.2 -
ポジティブ･コントロール
- WB: Recombinant Human MTHFD1 protein (ab116852), human cerebellum tissue lysate, rat and mouse brain tissue lysates and NIH3T3 cell lysate. IHC-P: Human tonsil tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab116615 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab116615の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 106 kDa.
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 106 kDa. 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. -
組織特異性
Detected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma. -
パスウェイ
One-carbon metabolism; tetrahydrofolate interconversion. -
配列類似性
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. -
ドメイン
This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 534296 Cow
- Entrez Gene: 476245 Dog
- Entrez Gene: 25902 Human
- Entrez Gene: 270685 Mouse
- Entrez Gene: 361472 Rat
- Omim: 611427 Human
- SwissProt: Q0VCR7 Cow
- SwissProt: Q6UB35 Human
see all -
別名
- 10-formyl-THF synthetase antibody
- C1TM_HUMAN antibody
- Formyltetrahydrofolate synthetase antibody
see all
画像
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Western blot - Anti-MTHFD1L antibody (ab116615)Anti-MTHFD1L antibody (ab116615) at 0.1 µg/ml + Human cerebellum tissue lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour.
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Western blot - Anti-MTHFD1L antibody (ab116615)All lanes : Anti-MTHFD1L antibody (ab116615) at 0.1 µg/ml
Lane 1 : NIH3T3 cell lysate in RIPA buffer
Lane 2 : Mouse brain tissue lysate in RIPA buffer
Lane 3 : Rat brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 106 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab116615 は 1 報の論文で使用されています。
- Leow MK et al. Activated brown adipose tissue releases exosomes containing mitochondrial methylene tetrahydrofolate dehydrogenase (NADP dependent) 1-like protein (MTHFD1L). Biosci Rep 42:N/A (2022). PubMed: 35502767