Anti-MTHFD1 抗体 (ab70203)
Key features and details
- Mouse polyclonal to MTHFD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTHFD1 antibody
MTHFD1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to MTHFD1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human MTHFD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- MTHFD1 transfected 293T cell lysate, human placenta cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70203の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 102 kDa).
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| 特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 102 kDa). |
ターゲット情報
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組織特異性
Ubiquitous. -
パスウェイ
One-carbon metabolism; tetrahydrofolate interconversion. -
関連疾患
Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500]. -
配列類似性
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. -
ドメイン
This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 4522 Human
- Omim: 172460 Human
- SwissProt: P11586 Human
- Unigene: 652308 Human
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別名
- 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase antibody
- C 1 tetrahydrofolate synthase cytoplasmic antibody
- C1 tetrahydrofolate synthase cytoplasmic antibody
see all
画像
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Western blot - Anti-MTHFD1 antibody (ab70203)Anti-MTHFD1 antibody (ab70203) at 1/500 dilution + human placenta at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Western blot - Anti-MTHFD1 antibody (ab70203)All lanes : Anti-MTHFD1 antibody (ab70203) at 1/500 dilution
Lane 1 : MTHFD1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (6)
ab70203 は 6 報の論文で使用されています。
- Tanaka K et al. Glioma cells require one-carbon metabolism to survive glutamine starvation. Acta Neuropathol Commun 9:16 (2021). PubMed: 33468252
- Di Marcantonio D et al. ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia. Mol Cell 81:2752-2764.e6 (2021). PubMed: 34081901
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746
- Yu H et al. Overexpression of MTHFD1 in hepatocellular carcinoma predicts poorer survival and recurrence. Future Oncol 15:1771-1780 (2019). PubMed: 30997850
- Zheng Y et al. Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells. Cell 175:1546-1560.e17 (2018). PubMed: 30500537
- Ducker GS et al. Human SHMT inhibitors reveal defective glycine import as a targetable metabolic vulnerability of diffuse large B-cell lymphoma. Proc Natl Acad Sci U S A 114:11404-11409 (2017). PubMed: 29073064
