Anti-MTG16 抗体 (ab129444)
Key features and details
- Rabbit polyclonal to MTG16
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTG16 antibody -
製品の詳細
Rabbit polyclonal to MTG16 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human MTG16 aa 603-653.
Database link: NP_005178 -
ポジティブ・コントロール
- Jurkat, 293T, HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7-8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129444の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 71 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Functions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation.
Isoform 2 functions as an A-kinase-anchoring protein. -
組織特異性
Widely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level). -
関連疾患
Note=A chromosomal aberration involving CBFA2T3 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein. -
配列類似性
Belongs to the CBFA2T family.
Contains 1 MYND-type zinc finger.
Contains 1 TAFH (NHR1) domain. -
細胞内局在
Nucleus > nucleolus. The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm and Nucleus > nucleoplasm. Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 863 Human
- Omim: 603870 Human
- SwissProt: O75081 Human
- Unigene: 513811 Human
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別名
- Cbfa2t3 antibody
- Ccore binding factor runt domain alpha subunit 2 translocated to 3 antibody
- ETO2 antibody
see all
画像
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All lanes : Anti-MTG16 antibody (ab129444) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Predicted band size: 71 kDa -
Detection of MTG16 by Western Blot of Immunprecipitate.
Lane 1: ab129444 at 1µg/ml staining MTG16 in HeLa whole cell lysate immunoprecipitated using ab129444 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab129444 は 1 報の論文で使用されています。
- Li J et al. CTCF: A novel fusion partner of ETO2 in a multiple relapsed acute myeloid leukemia patient. J Leukoc Biol 111:981-987 (2022). PubMed: 34622967