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AB228829

Anti-MTF2 抗体 - C-terminal

Anti-MTF2 antibody - C-terminal

  • JP Deleterious:医薬用外劇物

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(1 Publication)

Rabbit Polyclonal MTF2 antibody. C-terminal. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MTF2.

別名を表示する

PCL2, MTF2, Metal-response element-binding transcription factor 2, Metal regulatory transcription factor 2, Metal-response element DNA-binding protein M96, Polycomb-like protein 2, hPCl2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MTF2 antibody - C-terminal (AB228829)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTF2 antibody - C-terminal (AB228829)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MTF2 (green) using ab228829 at 1/250 dilution in ICC/IF.

Red : phalloidin, a cytoskeleton marker, diluted at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody - C-terminal (AB228829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF2 antibody - C-terminal (AB228829)

Paraffin-embedded mouse colon tissue stained for MTF2 using ab228829 at 1/1000 dilution in immunohistochemical analysis.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MTF2. The exact immunogen used to generate this antibody is proprietary information.

Q9Y483

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MTF2 also known as metal response element binding transcription factor 2 or M96 is a component of the Polycomb group (PcG) proteins. It's a protein involved in the regulation of gene expression via chromatin remodeling. MTF2 has a mass of approximately 110 kDa. It is expressed in various tissues with significant presence in the brain and embryonic stem cells where regulatory control of gene expression plays an important role in development.
Biological function summary

MTF2 acts mainly as a recruiter of Polycomb Repressive Complex (PRC) 2 to specific gene loci. This recruitment is essential for the methylation of histone H3 at lysine 27 (H3K27me3) a modification linked to transcriptional repression. MTF2’s role in gene silencing involves interaction with multiple other PcG proteins like EZH2 a component of PRC2 which further emphasizes its central position in maintaining the transcriptional repression needed for proper cell differentiation and development.

Pathways

MTF2 plays an important part in the regulation of the Wnt and Hedgehog signaling pathways. These pathways are significant in embryonic development and cell fate decisions. MTF2 interfaces with proteins such as SUZ12 within PRC2 which modulate repression activities by influencing chromatin structure in these pathways. Through these interactions MTF2 helps manage expression levels of downstream genes ultimately affecting cellular differentiation processes.

MTF2 has been implicated in several conditions related to dysregulation of gene expression. For example aberrant function or expression of MTF2 can contribute to cancers due in part to its role in controlling cell proliferation through gene silencing. Additionally mutations or dysfunctions in MTF2 have been connected to neurological disorders because of its high expression in brain tissues. The relationship between MTF2 and proteins in the PRC2 complex such as EZH2 is of particular interest in research related to these diseases as they collectively contribute to the maintenance of gene expression patterns critical to both normal development and disease states.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity (PubMed : 23142980, PubMed : 23228662, PubMed : 31959557). Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation (By similarity). Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells (By similarity). Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex (By similarity). In some conditions may act as an inhibitor of PRC2 activity : able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally (By similarity). Binds to the metal-regulating-element (MRE) of MT1A gene promoter (By similarity).
See full target information MTF2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

FEBS open bio : PubMed31984680

2020

BBOX1-AS1 contributes to colorectal cancer progression by sponging hsa-miR-361-3p and targeting SH2B1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiasheng Liu,Jie Zhu,Zhe Xiao,Xufeng Wang,Jianfei Luo
View all publications
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