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リコンビナントRabMAb

Anti-MTERF 抗体 [EPR14202] (ab181836)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)
  • Flow Cytometry (Intracellular) - Anti-MTERF antibody [EPR14202] (ab181836)
  • Anti-MTERF antibody [EPR14202] (ab181836)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14202] to MTERF
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MTERF antibody [EPR14202]
    MTERF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14202] to MTERF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-Pmore details
    適用なし: WB

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human liver tissue and Mouse muscle tissue. Flow Cyt (Intra): Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14202

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181836の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene; this region comprises a tridecamer sequence critical for directing accurate termination. Binds DNA along the major grove and promotes DNA bending and partial unwinding. Promotes base flipping. Probably requires one or more components for termination activity.

  • 配列類似性

    Belongs to the mTERF family.

  • ドメイン

    Contains nine structural repeats of about 35 residues, where each repeat contains three helices. The repeats form a left-handed superhelical assembly with a solenoid structure that wraps itself around DNA.

  • 翻訳後修飾

    Phosphoprotein with mostly four phosphate groups. While the DNA-binding activity is unaffected by the phosphorylation state, only the phosphorylated form of the protein is active for termination activity. Functioning seems to be regulated by phosphorylation.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • mitochondrial antibody
    • Mitochondrial transcription termination factor 1 antibody
    • mTERF antibody
    • MTERF_HUMAN antibody
    • Transcription termination factor antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)

    Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTERF antibody [EPR14202] (ab181836)

    Immunohistochemical analysis of paraffin embedded mouse muscle tissue sections labeling MTERF using ab181836 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-MTERF antibody [EPR14202] (ab181836)
    Flow Cytometry (Intracellular) - Anti-MTERF antibody [EPR14202] (ab181836)

    Intracellular Flow Cytometry analysis of Jurkat cells labeling MTERF using ab181836 at a 1/50 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde. 

     

     

  • Anti-MTERF antibody [EPR14202] (ab181836)
    Anti-MTERF antibody [EPR14202] (ab181836)

参考文献 (0)

ab181836 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181836 は論文での使用が確認できていません。

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