AB171073

Anti-MTA2/PID 抗体 [EPR8537(2)]

Anti-MTA2/PID antibody [EPR8537(2)]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal MTA2/PID antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

別名を表示する

MTA1L1, PID, MTA2, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1, p53 target protein in deacetylase complex, MTA1-L1 protein

5 Images

Flow Cytometry (Intracellular) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Intracellular flow cytometric analysis of permeabilized Hela cells labeling MTA2/PID with ab171073 at 1/100 dilution (red)or a rabbit IgG negative (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling MTA2/PID with ab171073 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Immunofluorescent staining of HeLa cells labeling MTA2/PID with ab171073 at 1/250 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MTA2/PID with ab171073 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

All lanes:

Western blot - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Predicted band size: 75 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8537(2)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval is recommended", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval is recommended", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Antigen retrieval is recommended", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MTA2 also known as metastasis-associated protein 2 is a component of the nucleosome remodeling and deacetylase (NuRD) complex weighing approximately 77 kDa. MTA2 is widely expressed throughout the human body including in vital organs and tissues such as the liver brain and lung. It plays a significant role in regulating gene expression by modifying chromatin structure. This protein often localizes in the nucleus where it can regulate various cellular processes.

Biological function summary

MTA2 influences the suppression and activation of gene transcription. It acts as an integral part of the NuRD complex which combines chromatin remodeling and histone deacetylase activities. Through this role MTA2 helps control cellular differentiation proliferation and development. By modulating epigenetic states MTA2 can impact cellular responses to external stimuli helping cells adapt to changes in their environment.

Pathways

MTA2 engages in cellular signaling pathways including the Notch signaling and Wnt signaling pathways. These pathways are important for regulating cell fate decisions. MTA2 interacts with other proteins such as HDAC1 and HDAC2 to exert its influence on transcriptional repression and gene silencing. The NuRD complex of which MTA2 is part serves as a critical mediator in these pathways highlighting its importance in cellular homeostasis.

MTA2 participation links it to cancer progression and metastasis. It shows overexpression in several cancers including breast and liver cancer where it correlates with more aggressive tumor behavior and poor prognosis. Interestingly MTA2 also associates with prostate cancer through its interaction with the androgen receptor influencing cancer cell survival and proliferation. Identifying MTA2’s connections with these conditions has pointed to potential therapeutic targets within these molecular pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May function as a transcriptional coregulator (PubMed : 16428440, PubMed : 28977666). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666).

See full target information MTA2

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e113448 PubMed37737560

2023

BCL11B and the NuRD complex cooperatively guard T-cell fate and inhibit OPA1-mediated mitochondrial fusion in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liao,Yi Wu,Le Qin,Zhiwu Jiang,Shixue Gou,Linfu Zhou,Qilan Hong,Yao Li,Jingxuan Shi,Yao Yao,Liangxue Lai,Yangqiu Li,Pentao Liu,Jean Paul Thiery,Dajiang Qin,Thomas Graf,Xingguo Liu,Peng Li

Biomolecules 13: PubMed37371463

2023

Prognostic, Immunological, and Mutational Analysis of MTA2 in Pan-Cancer and Drug Screening for Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xueshan Huang,Jingyi Tan,Mei Chen,Weirang Zheng,Shanyang Zou,Xiaoxia Ye,Yutong Li,Minhua Wu

Pharmacology 106:573-587 PubMed34488224

2021

Long Noncoding RNA TTC39A-AS1 Promotes Breast Cancer Tumorigenicity by Sponging MicroRNA-483-3p and Thereby Upregulating MTA2.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohui Zhou,Ping Yang,Binming Zhang,Maohui Yao,Yali Jia,Na Li,Huimin Liu,Haiya Bai,Xiaojun Gong

Cancers 11: PubMed31771219

2019

MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Syuan Chen,Tung-Wei Hung,Shih-Chi Su,Chia-Liang Lin,Shun-Fa Yang,Chu-Che Lee,Chang-Fang Yeh,Yi-Hsien Hsieh,Jen-Pi Tsai

Science advances 3:e1700298 PubMed28560351

2017

Global increase in replication fork speed during a p57-regulated erythroid cell fate switch.

Applications

Unspecified application

Species

Unspecified reactive species

Yung Hwang,Melinda Futran,Daniel Hidalgo,Ramona Pop,Divya Ramalingam Iyer,Ralph Scully,Nicholas Rhind,Merav Socolovsky

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTA2/PID 抗体 [EPR8537(2)]

Anti-MTA2/PID antibody [EPR8537(2)]

Flow Cytometry (Intracellular) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

Western blot - Anti-MTA2/PID antibody [EPR8537(2)] (AB171073)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise