Anti-MTA1 抗体 (ab71153)
Key features and details
- Rabbit polyclonal to MTA1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTA1 antibody
MTA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MTA1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis, Platypus
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免疫原
A synthetic peptide corresponding to a region between residue 665 and the C-terminus (residue 715) of MTA1 (NP_004680.1).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71153の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 35, 81 kDa (predicted molecular weight: 81 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P | (5) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 35, 81 kDa (predicted molecular weight: 81 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER. -
組織特異性
Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual. -
配列類似性
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain. -
発生段階
Highly expressed in metastatic cells. -
ドメイン
Isoform Short contains a Leu-Arg-Ile-Leu-Leu motif (ER binding motif). -
細胞内局在
Cytoplasm and Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9112 Human
- Entrez Gene: 116870 Mouse
- Entrez Gene: 64520 Rat
- Omim: 603526 Human
- SwissProt: Q13330 Human
- SwissProt: Q8K4B0 Mouse
- SwissProt: Q62599 Rat
- Unigene: 525629 Human
see all -
別名
- Metastasis associated 1 antibody
- Metastasis associated gene 1 antibody
- Metastasis associated gene 1 protein antibody
see all
画像
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Western blot - Anti-MTA1 antibody (ab71153)All lanes : Anti-MTA1 antibody (ab71153) at 1/2000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : Mta1 CRISPR-Cas9 edited HEK-293 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 80-85 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-MTA1 antibody staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab71153 was shown to bind specifically to MTA1. A band was observed at 80/85 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in Mta1 CRISPR-Cas9 edited cell line ab277164 (CRISPR-Cas9 edited cell lysate ab277206). The band observed in the CRISPR-Cas9 edited lysate lane below 80/85 kDa is likely to represent a truncated form of MTA1. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and Mta1 CRISPR-Cas9 edited HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Western blot - Anti-MTA1 antibody (ab71153)All lanes : Anti-MTA1 antibody (ab71153) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA1 antibody (ab71153)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling MTA1 with ab71153 at 1/1000 (0.2 µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-MTA1 antibody (ab71153)Immunoprecipitation/ Western Blot of MTA1
Lane 1: ab71153 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Subsequent WB detection was performed using 1 µg/ml ab71153.
Chemiluminescence with an exposure time of 1 second.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (43)
ab71153 は 43 報の論文で使用されています。
- Ishikawa M et al. MTA1, a metastasis‑associated protein, in endothelial cells is an essential molecule for angiogenesis. Mol Med Rep 25:N/A (2022). PubMed: 34779499
- Dufour CR et al. The mTOR chromatin-bound interactome in prostate cancer. Cell Rep 38:110534 (2022). PubMed: 35320709
- Zhou Y et al. Upregulation of MTA1 in Colon Cancer Drives A CD8+ T Cell-Rich But Classical Macrophage-Lacking Immunosuppressive Tumor Microenvironment. Front Oncol 12:825783 (2022). PubMed: 35350571
- Ma K et al. Correlation between metastasis-associated gene 1 expression and tumor-associated macrophages in non-small cell lung cancer. Neoplasma 69:1092-1100 (2022). PubMed: 35951452
- Guo N & Zhang J Interleukin-17 promotes the development of ovarian cancer through upregulation of MTA1 expression. Am J Cancer Res 12:5646-5656 (2022). PubMed: 36628289