Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-MT2-MMP 抗体 [MM0028-5D11] (ab56308)

Submit a review Q&A (2)References (1)
Western blot - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)

Key features and details

  • Mouse monoclonal [MM0028-5D11] to MT2-MMP
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-MT2-MMP antibody [MM0028-5D11]

  • 製品の詳細

    Mouse monoclonal [MM0028-5D11] to MT2-MMP

  • 由来種

    Mouse

  • 特異性

    ab56308 recognizes human MT2-MMP in western blot without cross reactivity with human MMP14/16. Reactivity with MT2-MMP in other species has not been tested.

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human MT2-MMP.

  • ポジティブ･コントロール

    • WB: Human placenta tissue lysate. IHC-P: Human placenta tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56308の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/400 - 1/1000. Predicted molecular weight: 76 kDa.
IHC-P
1/50 - 1/200.
特記事項
WB
1/400 - 1/1000. Predicted molecular weight: 76 kDa.
IHC-P
1/50 - 1/200.

ターゲット情報

  • 機能

    Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.

  • 組織特異性

    Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.

  • 配列類似性

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.

  • ドメイン

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 翻訳後修飾

    The precursor is cleaved by a furin endopeptidase.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AI503551 antibody
      • Matrix metallopeptidase 15 membrane inserted antibody
      • Matrix metalloproteinase 15 antibody
      • Matrix metalloproteinase-15 antibody
      • Membrane type 2 matrix metalloproteinase antibody
      • Membrane type matrix metalloproteinase 2 antibody
      • Membrane-type matrix metalloproteinase 2 antibody
      • Membrane-type-2 matrix metalloproteinase antibody
      • MMP 15 antibody
      • MMP-15 antibody
      • MMP15 antibody
      • MMP15_HUMAN antibody
      • MT MMP 2 antibody
      • MT-MMP 2 antibody
      • MT2 MMP antibody
      • MT2-MMP antibody
      • MT2MMP antibody
      • MTMMP 2 antibody
      • MTMMP2 antibody
      • SMCP 2 antibody
      • SMCP-2 antibody
      • SMCP2 antibody
      see all

    画像

    • Western blot - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
      Western blot - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
      Anti-MT2-MMP antibody [MM0028-5D11] (ab56308) at 1/400 dilution + Human placenta tissue lysate

      Predicted band size: 76 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT2-MMP antibody [MM0028-5D11] (ab56308)

      ab56308 at 1/200 dilution, staining MT2-MMP in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

    参考文献 (1)

    ab56308 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56308 は 1 報の論文で使用されています。

    • Yi G  et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

     Hi,   I’m wondering how long you suggest an antibody lasts for – I purchased several antibodies about eight months ago and none of them came with an expiry date, the info sheets just say “store at -20 long term”.  These antibodies are:   AB38971 Rabbit Polyclonal to MMP14 - Hinge region Lot # GR12166 AB56308 Mouse Monoclonal [MM0028-5D11] to MMP15 Lot #GR40525 AB73877 Rabbit Polyclonal to MMP16 Lot # GR30734 AB51075 Rabbit Monoclonal [EP1270Y] to MMP17 Lot # GR19885 AB39031 Rabbit Polyclonal to MMP25 - Hinge region Lot # 275516   Thank you,      

    Read More
    Answer

    Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly. For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols). I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Read More

    Question

    Dear Abcam, I was hoping that you could provide me with some more detail about the mouse monoclonal antibody targeting MMP-15 (AB56308). I am after some information regarding the immunogen used to create this antibody or at least the region of MMP-15 that it targets. Thank you for your help.

    Read More
    Answer

    Thank you for contacting us. I was informed that it was tested to pick up the extracellular domain of the recombinant protein. Unfortunately, we do not have the detailed epitope information available. We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com