Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) 抗体 [EP2123Y] (ab76579)
![Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)](https://www.abcam.co.jp/ps/products/76/ab76579/Images/ab76579-92080-anti-mst4-mst3-stk25-phospho-t174-t178-t190-antibody-ep2123y-western-blot.gif "Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2123Y] to MST4 + MST3 + STK25 (phospho T174 + T178 + T190)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y]
MST4 + MST3 + STK25 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2123Y] to MST4 + MST3 + STK25 (phospho T174 + T178 + T190) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa cell lysates treated with calyculin A.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2123Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76579の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
ICC: 1/100 - 1/250.
WB: 1/1000 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: MST3, 49 kDa; MST4, 47 kDa & STK25, 48 kDa).
Is unsuitable for Flow Cyt, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: MST3, 49 kDa; MST4, 47 kDa & STK25, 48 kDa).
Is unsuitable for Flow Cyt, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Novel human Ste20-related kinase Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation. It is pro apoptotic and is highly expressed in placenta, thymus, and peripheral blood leukocytes. Interaction with Golgi matrix protein GOLGA2 results in autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. This may also be activated by C terminal cleavage. MST3 or Mammalian Sterile 20-like kinase 3 is a member of the germinal center kinase-III family. MST3 contains a conserved kinase domain at its NH(2)-terminus and a regulatory domain at its COOH-terminus. Caspase-mediated cleavage of the regulatory domain of MST3 activates its intrinsic kinase activity and leads to nuclear translocation. Expression of COOH-terminal truncated MST3 in cells results in DNA fragmentation and induction of apoptosis. It can inhibit cell migration in a fashion dependent on autophosphorylation and can regulate paxillin phosphorylation through tyrosine phosphatase PTP-PEST. Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade. -
細胞内局在
STK25 and MST4: Cytoplasmic, Golgi Apparatus MST3: Cytoplasmic -
参照データベース
- Entrez Gene: 10494 Human
- Entrez Gene: 51765 Human
- Entrez Gene: 8428 Human
- Omim: 300547 Human
- Omim: 602255 Human
- Omim: 604984 Human
- SwissProt: O00506 Human
- SwissProt: Q9P289 Human
see all -
別名
- 0610042I06Rik antibody
- 1500019J11Rik antibody
- AU018434 antibody
see all
画像
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Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)All lanes : Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579) at 1/2000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 47-49 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
![Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)](https://www.abcam.co.jp/ps/products/76/ab76579/Images/ab76579-2-benefits-of-recombinant-antibodies.png)
Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab76579 は 7 報の論文で使用されています。
- Wagner M et al. Cellular Mechanisms of the Anti-Arrhythmic Effect of Cardiac PDE2 Overexpression. Int J Mol Sci 22:N/A (2021). PubMed: 34062838
- Rodriguez-Cupello C et al. The STRIPAK Complex Regulates Response to Chemotherapy Through p21 and p27. Front Cell Dev Biol 8:146 (2020). PubMed: 32258031
- An L et al. MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway. J Exp Med 217:N/A (2020). PubMed: 32271880
- Mardakheh FK et al. RHO binding to FAM65A regulates Golgi reorientation during cell migration. J Cell Sci 129:4466-4479 (2016). WB ; Human . PubMed: 27807006
- Costa B et al. STK25 Protein Mediates TrkA and CCM2 Protein-dependent Death in Pediatric Tumor Cells of Neural Origin. J Biol Chem 287:29285-9 (2012). WB ; Human . PubMed: 22782892
- Gordon J et al. Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3. BMC Biochem 12:54 (2011). WB . PubMed: 21985334
- Cornils H et al. Human NDR kinases control G(1)/S cell cycle transition by directly regulating p21 stability. Mol Cell Biol 31:1382-95 (2011). PubMed: 21262772