Anti-MSS1 抗体 (ab3322)
Key features and details
- Rabbit polyclonal to MSS1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MSS1 antibody
MSS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MSS1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human MSS1. Human recombinant proteasome 19S subunit S7.
-
ポジティブ･コントロール
- WB: HeLa cell lysate. ICC/IF: SK-OV-3 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3322の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/250.
|
|
| WB |
1/1000.
By Western blot, this antibody detects a 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate. |
| 特記事項 |
|---|
|
ICC/IF
1/250. |
|
WB
1/1000. By Western blot, this antibody detects a 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate. |
ターゲット情報
-
機能
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation. -
配列類似性
Belongs to the AAA ATPase family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 5701 Human
- Omim: 154365 Human
- SwissProt: P35998 Human
- Unigene: 437366 Human
-
別名
- 26S protease complex antibody
- 26S protease regulatory subunit 7 antibody
- 26S proteasome AAA-ATPase subunit RPT1 antibody
see all
画像
-
Western blot - Anti-MSS1 antibody (ab3322)
Western blot of MSS1 on HeLa cell lysate using ab3322.
-
Immunocytochemistry/ Immunofluorescence - Anti-MSS1 antibody (ab3322)Immunofluorescence analysis of MSS1 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MSS1 Rabbit Polyclonal Antibody (ab3322) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (6)
ab3322 は 6 報の論文で使用されています。
- Auvin S et al. A molecule inducing androgen receptor degradation and selectively targeting prostate cancer cells. Life Sci Alliance 2:N/A (2019). PubMed: 31431473
- Maganti N et al. Nonproteolytic roles of 19S ATPases in transcription of CIITApIV genes. PLoS One 9:e91200 (2014). Human . PubMed: 24625964
- Sui Z et al. Fusion-Related Host Proteins Are Actively Regulated by NA during Influenza Infection as Revealed by Quantitative Proteomics Analysis. PLoS One 9:e105947 (2014). WB ; Dog . PubMed: 25153908
- Anderson DD et al. Competition between Sumoylation and Ubiquitination of Serine Hydroxymethyltransferase 1 Determines Its Nuclear Localization and Its Accumulation in the Nucleus. J Biol Chem 287:4790-9 (2012). ICC/IF ; Human . PubMed: 22194612
- Amazit L et al. Ligand-Dependent Degradation of SRC-1 Is Pivotal for Progesterone Receptor Transcriptional Activity. Mol Endocrinol 25:394-408 (2011). ICC/IF ; Human . PubMed: 21273440
- Rousseau E et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human, Rat . PubMed: 18986984
