Anti-MSP/MST1 抗体 [EPR22184-180] - BSA and Azide free (ab245826)
![Western blot - Anti-MSP/MST1 antibody [EPR22184-180] - BSA and Azide free (ab245826)](https://www.abcam.co.jp/ps/products/245/ab245826/Images/ab245826-1-245190WB2.jpg "Western blot - Anti-MSP/MST1 antibody [EPR22184-180] - BSA and Azide free (ab245826)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22184-180] to MSP/MST1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MSP/MST1 antibody [EPR22184-180] - BSA and Azide free
MSP/MST1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22184-180] to MSP/MST1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-Fr,IHC-P or IP -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse serum.
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特記事項
ab245826 is the carrier-free version of ab245190.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22184-180 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245826の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 81, 60 kDa (predicted molecular weight: 81 kDa).
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 81, 60 kDa (predicted molecular weight: 81 kDa). |
ターゲット情報
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機能
Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. -
配列類似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
May be cleaved after Arg-483, to yield two chains held together by disulfide bonds, or two separate polypeptides. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4485 Human
- Entrez Gene: 15235 Mouse
- Omim: 142408 Human
- SwissProt: P26927 Human
- SwissProt: P26928 Mouse
- Unigene: 349110 Human
- Unigene: 512587 Human
- Unigene: 8369 Mouse
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別名
- D3F15S2 antibody
- DNF15S2 antibody
- Hepatocyte growth factor like protein alpha chain antibody
see all
画像
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Western blot - Anti-MSP/MST1 antibody [EPR22184-180] - BSA and Azide free (ab245826)All lanes : Anti-MSP/MST1 antibody [EPR22184-180] (ab245190) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 60, 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Pro-MSP is proteolytically cleaved into alpha and beta chain. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8393443;8609228;7732008;11856768).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245190).
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![Anti-MSP/MST1 antibody [EPR22184-180] - BSA and Azide free (ab245826)](https://www.abcam.co.jp/ps/products/245/ab245826/Images/ab245826-2-benefits-of-recombinant-antibodies.png)
Anti-MSP/MST1 antibody [EPR22184-180] - BSA and Azide free (ab245826)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab245826 は論文での使用が確認できていません。
