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Anti-MSK2 / RSK-B 抗体 (ab42101)

Reviews (2)Q&A (1)References (2)
Western blot - Anti-MSK2 / RSK-B antibody (ab42101)
  • Immunocytochemistry/ Immunofluorescence - Anti-MSK2 / RSK-B antibody (ab42101)

Key features and details

  • Rabbit polyclonal to MSK2 / RSK-B
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-MSK2 / RSK-B antibody
    MSK2 / RSK-B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to MSK2 / RSK-B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human MSK2/ RSK-B.

    Immunogen の所有権に関して (Peptide available as ab42280.)

  • ポジティブ･コントロール

    • This antibody gave a positive signal in the following cell human lysates: HeLa Nuclear Lysate and HepG2 Whole Cell Lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab42101の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ICC/IF
Use a concentration of 1 - 5 µg/ml.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ICC/IF
Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能

    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 90 kDa ribosomal protein S6 kinase 4 antibody
      • EC 2.7.11.1 antibody
      • KS6A4_HUMAN antibody
      • Mitogen and stress activated protein kinase 2 antibody
      • Nuclear mitogen and stress activated protein kinase 2 antibody
      • Nuclear mitogen- and stress-activated protein kinase 2 antibody
      • Ribosomal protein kinase B antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 4 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody
      • Ribosomal protein S6 kinase alpha 4 antibody
      • Ribosomal protein S6 kinase alpha-4 antibody
      • RPS6KA4 antibody
      • RSKB antibody
      • S6K-alpha-4 antibody
      see all

    画像

    • Western blot - Anti-MSK2 / RSK-B antibody (ab42101)
      Western blot - Anti-MSK2 / RSK-B antibody (ab42101)
      All lanes : Anti-MSK2 / RSK-B antibody (ab42101) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 86 kDa
      Observed band size: 86 kDa
      Additional bands at: 150 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-MSK2 / RSK-B antibody (ab42101)
      Immunocytochemistry/ Immunofluorescence - Anti-MSK2 / RSK-B antibody (ab42101)
      ICC/IF image of ab42101 stained MCF7 cells. The cells were 4%PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42101, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4%PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 5ug/ml.

    参考文献 (2)

    ab42101 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab42101 は 2 報の論文で使用されています。

    • Kang JI & Ahn BY HCV-induced PKR activation is stimulated by the mitogen- and stress-activated protein kinase MSK2. Biochem Biophys Res Commun 407:248-53 (2011). WB ; Human . PubMed: 21385567
    • Raymond B  et al. Anthrax lethal toxin impairs IL-8 expression in epithelial cells through inhibition of histone H3 modification. PLoS Pathog 5:e1000359 (2009). WB ; Human . PubMed: 19343203

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-MSK2 / RSK-B antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat Schwann cell)
    Loading amount
    1.2e+006 cells
    Specification
    Rat Schwann cell
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10% gel)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 11 2011

    Western blot abreview for Anti-MSK2 / RSK-B antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Huh-7)
    Loading amount
    30 µg
    Specification
    Huh-7
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. JU-IL KANG

    Verified customer

    投稿 May 19 2009

    Question

    100% Testing Discount for ab42101 to test on rat tissue in WB.

    Read More
    Answer

    DISCOUNT CODE: ******* Expiration date: ******* I am very pleased to hear you would like to accept our offer and test ab42101 inWB on rat tissue. This code will give you 1 free [primary antibody before the expiration date. To redeem this offer, please submit an Abreview for wesetrn blot on rat tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More

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