Anti-MSK1 (phospho S376) 抗体 [E375] (ab32190)
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)](https://www.abcam.co.jp/ps/products/32/ab32190/Images/ab32190-261275-anti-msk1-phospho-s376-antibody-e375-western-blot.jpg "Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E375] to MSK1 (phospho S376)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MSK1 (phospho S376) antibody [E375]
MSK1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E375] to MSK1 (phospho S376) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma. ICC/IF: HepG2 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E375 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32190の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa).
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| IHC-P | (5) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF | (1) |
1/500.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/500. |
追加情報
Is unsuitable for Flow Cyt.
ターゲット情報
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機能
Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). -
組織特異性
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻訳後修飾
Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. -
細胞内局在
Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 9252 Human
- Entrez Gene: 314384 Rat
- Omim: 603607 Human
- SwissProt: O75582 Human
- Unigene: 510225 Human
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別名
- 90 kDa ribosomal protein S6 kinase 5 antibody
- EC 2.7.11.1 antibody
- KS6A5_HUMAN antibody
see all
画像
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Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution
Lane 1 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 (phosphor S376) peptide
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 non-phospho peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 10 secondsBlocking/Diluting buffer: 5% NFDM/TBST
Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1. -
![Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)](https://www.abcam.co.jp/ps/products/32/ab32190/Images/ab32190-451289-anti-msk1-phospho-s376-antibody-e375-immunocytochemistry-hepg2human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37℃ 1h). -
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)](https://www.abcam.co.jp/ps/products/32/ab32190/Images/ab32190-261274-anti-msk1-phospho-s376-antibody-e375-western-blot.jpg)
Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution
Lane 1 : Untreated HEK293 (human embryonic kidney) whole cell lysate
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 5 secondsBlocking/Diluting buffer 5% NFDM/TBST.
Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1. -
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)](https://www.abcam.co.jp/ps/products/32/ab32190/Images/ab32190-44184-anti-msk1-phospho-s376-antibody-e375-western-blot.jpg)
Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution
Lane 1 : Untreated K562 cell lysate
Lane 2 : K562 cell lysate treated with Alkaline
Predicted band size: 90 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)](https://www.abcam.co.jp/ps/products/32/ab32190/Images/ab32190-44183-anti-msk1-phospho-s376-antibody-e375-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] (ab32190)Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MSK1 (phospho S376) antibody [E375] (ab32190)](https://www.abcam.co.jp/ps/products/32/ab32190/Images/ab32190-5-benefits-of-recombinant-antibodies.png)
Anti-MSK1 (phospho S376) antibody [E375] (ab32190)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab32190 は 4 報の論文で使用されています。
- Li J et al. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Nat Commun 12:896 (2021). PubMed: 33563994
- Khan SA et al. p38-MAPK/MSK1-mediated overexpression of histone H3 serine 10 phosphorylation defines distance-dependent prognostic value of negative resection margin in gastric cancer. Clin Epigenetics 8:88 (2016). PubMed: 27588146
- Rylander D et al. Pharmacological modulation of glutamate transmission in a rat model of L-DOPA-induced dyskinesia: effects on motor behavior and striatal nuclear signaling. J Pharmacol Exp Ther 330:227-35 (2009). PubMed: 19357321
- Westin JE et al. Spatiotemporal pattern of striatal ERK1/2 phosphorylation in a rat model of L-DOPA-induced dyskinesia and the role of dopamine D1 receptors. Biol Psychiatry 62:800-10 (2007). IHC ; Rat . PubMed: 17662258