AB32190

Anti-MSK1 (phospho S376) 抗体 [E375]

Name: Anti-MSK1 (phospho S376) 抗体 [E375] (ab32190) | Abcam
Brand: Abcam Limited
SKU: AB32190
Price: 44000 JPY
Availability: InStock
Rating: 5 (10 reviews)

Anti-MSK1 (phospho S376) antibody [E375]

RabMAb
Recombinant
詳細を見る

(10 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal MSK1 phospho S376 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 5 publications.

別名を表示する

MSK1, RPS6KA5, Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

ab32190 at a dilution of 1/50 staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 Alexa Fluor^® 488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.

Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37°C 1h).

Lab

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

Blocking/Diluting buffer : 5% NFDM/TBST

Observed band : 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

All lanes:

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

Lane 1:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg

Lane 2:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg with MSK1 (phosphor S376) peptide

Lane 3:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg with MSK1 non-phospho peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 90 kDa

false

Exposure time: 10s

Unknown

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

All lanes:

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution

Lane 1:

Untreated K562 cell lysate

Lane 2:

K562 cell lysate treated with Alkaline

Predicted band size: 90 kDa

Observed band size: 87 kDa

false

Lab

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

Blocking/Diluting buffer 5% NFDM/TBST.

Observed band : 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

All lanes:

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

Lane 1:

Untreated HEK293 (human embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate at 10 µg

Lane 3:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 90 kDa

false

Exposure time: 5s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

E375

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

製品の詳細

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The mitogen- and stress-activated protein kinase 1 known as MSK1 or RPS6KA5 acts as a serine/threonine kinase. It has a mass of approximately 92 kDa. MSK1 phosphorylates various substrates including proteins involved in gene expression regulation. Researchers find MSK1 expressed mainly in the brain but it appears also in kidneys and lungs. Phosphorylation by MSK1 influences transcription factors like CREB and ATF1 affecting cellular responses to stimuli.

Biological function summary

MSK1 influences cell proliferation and survival. It operates as part of the MAPK signaling pathway driven by extracellular signals. MSK1 activation occurs through ERK1/2 and p38 MAPK therefore linking it to essential cellular processes. As a kinase MSK1 phosphorylates downstream targets contributing to inflammatory responses apoptosis and gene expression modulation. MSK1 often partners with other proteins to form complexes enhancing its regulatory capabilities.

Pathways

MSK1 plays a role in both the MAPK and NF-kB pathways. These pathways contribute to controlling cell growth and immune responses. In the MAPK pathway the involvement of MSK1 influences further regulation of transcription factors. In connection with the NF-kB pathway MSK1 can regulate NF-kB activity promoting gene expression related to inflammation. Related proteins in these pathways include ERK1/2 which activate MSK1 enhancing the kinase's function in these critical cellular circuits.

MSK1 links to various conditions including cancer and neurodegenerative diseases. The dysregulation of MSK1 activity is associated with processes like uncontrolled cell proliferation and abnormal inflammation factors that contribute to tumor development and progression. In neurodegenerative diseases changes in MSK1 activity disrupt neuronal survival pathways affecting cognitive functions. MSK1 connects with proteins like CREB influencing the expression of genes relevant to these diseases' development.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed : 11909979, PubMed : 12569367, PubMed : 12763138, PubMed : 18511904, PubMed : 9687510, PubMed : 9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed : 11909979, PubMed : 9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed : 12628924, PubMed : 18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed : 12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed : 12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed : 12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed : 15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed : 12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed : 12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed : 12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed : 25851810).

See full target information RPS6KA5 phospho S376

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1572878 PubMed40356959

2025

Apigenin promotes melanogenesis and melanosome transport through the c-KIT/Raf-1/MAPK/CREB pathway in HEMCs.

Applications

Unspecified application

Species

Unspecified reactive species

Jinpeng Lv,Duo Meng,Huansha Zhang,Wenhui Xu,Xiaohong An,Chuanwei Yin,Kun Zou,Rongyin Gao

Nature communications 12:896 PubMed33563994

2021

Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Barun Mahata,Mario Escobar,Jacob Goell,Kaiyuan Wang,Pranav Khemka,Isaac B Hilton

Clinical epigenetics 8:88 PubMed27588146

2016

p38-MAPK/MSK1-mediated overexpression of histone H3 serine 10 phosphorylation defines distance-dependent prognostic value of negative resection margin in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shafqat Ali Khan,Ramchandra Amnekar,Bharat Khade,Savio George Barreto,Mukta Ramadwar,Shailesh V Shrikhande,Sanjay Gupta

The Journal of pharmacology and experimental thera 330:227-35 PubMed19357321

2009

Pharmacological modulation of glutamate transmission in a rat model of L-DOPA-induced dyskinesia: effects on motor behavior and striatal nuclear signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Daniella Rylander,Alessandra Recchia,Flora Mela,Andrzej Dekundy,Wojciech Danysz,M Angela Cenci

Biological psychiatry 62:800-10 PubMed17662258

2007

Spatiotemporal pattern of striatal ERK1/2 phosphorylation in a rat model of L-DOPA-induced dyskinesia and the role of dopamine D1 receptors.

Applications

IHC

Species

Rat

Jenny E Westin,Linda Vercammen,Elissa M Strome,Christine Konradi,M Angela Cenci

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSK1 (phospho S376) 抗体 [E375]

Anti-MSK1 (phospho S376) antibody [E375]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (AB32190)

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise