Anti-MSK1 (phospho S360) 抗体 [EP1888Y] (ab81294)
![Western blot - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)](https://www.abcam.co.jp/ps/products/81/ab81294/Images/MSK1-Primary-antibodies-ab81294-1.JPG "Western blot - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)")
Key features and details
- Rabbit monoclonal [EP1888Y] to MSK1 (phospho S360)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MSK1 (phospho S360) antibody [EP1888Y]
MSK1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1888Y] to MSK1 (phospho S360) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HEK293 cell lyasate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1888Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81294の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 90 kDa.
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| ICC/IF |
1/100 - 1/250.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Predicted molecular weight: 90 kDa. |
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ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for Flow Cyt or IHC-P.
ターゲット情報
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機能
Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). -
組織特異性
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻訳後修飾
Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. -
細胞内局在
Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 9252 Human
- Entrez Gene: 73086 Mouse
- Entrez Gene: 314384 Rat
- Omim: 603607 Human
- SwissProt: O75582 Human
- SwissProt: Q8C050 Mouse
- Unigene: 510225 Human
- Unigene: 220417 Mouse
see all -
別名
- 90 kDa ribosomal protein S6 kinase 5 antibody
- EC 2.7.11.1 antibody
- KS6A5_HUMAN antibody
see all
画像
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Western blot - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294) at 1/20000 dilution
Lane 1 : HEK293 cell lyasate, untreated,
Lane 2 : HEK293 cell lyasate treated with AP.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit. at 1/2000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)](https://www.abcam.co.jp/ps/products/81/ab81294/Images/MSK1-Primary-antibodies-ab81294-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294)ICC/IF image of ab81294 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81294, neat) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab81294 は 9 報の論文で使用されています。
- Ning B et al. MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats. Exp Ther Med 21:364 (2021). PubMed: 33732337
- Portelinha A et al. ASN007 is a selective ERK1/2 inhibitor with preferential activity against RAS-and RAF-mutant tumors. Cell Rep Med 2:100350 (2021). PubMed: 34337566
- Hou M et al. Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. Infect Immun 87:N/A (2019). PubMed: 30396897
- Ning B et al. MSK1 downregulation is associated with neuronal and astrocytic apoptosis following subarachnoid hemorrhage in rats. Oncol Lett 14:2940-2946 (2017). PubMed: 28927047
- Wiersma M et al. Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes. Epigenetics Chromatin 9:52 (2016). WB ; Human . PubMed: 27895715
- Ufelmann H & Schrenk D Nodularin-triggered apoptosis and hyperphosphorylation of signaling proteins in cultured rat hepatocytes. Toxicol In Vitro 29:16-26 (2015). PubMed: 25192816
- Fieblinger T et al. Mechanisms of dopamine D1 receptor-mediated ERK1/2 activation in the parkinsonian striatum and their modulation by metabotropic glutamate receptor type 5. J Neurosci 34:4728-40 (2014). PubMed: 24672017
- Harouz H et al. Shigella flexneri targets the HP1? subcode through the phosphothreonine lyase OspF. EMBO J 33:2606-22 (2014). ICC/IF ; Human . PubMed: 25216677
- Hamdi MM & Mutungi G Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in mouse skeletal muscle fibres through an ERK1/2-dependent mechanism. J Physiol 589:3623-40 (2011). WB ; Mouse . PubMed: 21606113