Anti-MSH3 抗体 (ab69619)
Key features and details
- Rabbit polyclonal to MSH3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MSH3 antibody
MSH3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MSH3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived from an internal region of human MSH3.
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ポジティブ･コントロール
- Extracts from HUVEC cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69619の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa). |
ターゲット情報
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機能
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. -
関連疾患
Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089]. -
配列類似性
Belongs to the DNA mismatch repair mutS family. MSH3 subfamily. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 4437 Human
- Omim: 600887 Human
- SwissProt: P20585 Human
- Unigene: 280987 Human
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別名
- Divergent upstream protein antibody
- DNA mismatch repair protein antibody
- DNA mismatch repair protein Msh 3 antibody
see all
画像
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Western blot - Anti-MSH3 antibody (ab69619)All lanes : Anti-MSH3 antibody (ab69619) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab69619 は 3 報の論文で使用されています。
- Ferreira da Silva J et al. Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair. Nat Commun 13:760 (2022). PubMed: 35140211
- Keogh N et al. MutSß abundance and Msh3 ATP hydrolysis activity are important drivers of CTG•CAG repeat expansions. Nucleic Acids Res 45:10068-10078 (2017). PubMed: 28973443
- Zhao J et al. Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks. Nucleic Acids Res 37:4420-9 (2009). WB ; Human . PubMed: 19468048