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リコンビナントRabMAb

Anti-MRPP3 抗体 [EPR14321-30] (ab185942)

Submit a review Submit a question References (1)
Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
  • Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
  • Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
  • Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
  • Anti-MRPP3 antibody [EPR14321-30] (ab185942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14321-30] to MRPP3
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MRPP3 antibody [EPR14321-30]
    MRPP3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14321-30] to MRPP3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562, 293, HeLa and U87-MG cell lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185942の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 56-67 kDa (predicted molecular weight: 67 kDa).

Based on the sequence analysis by BLAST, ab185942 recognizes three isoforms with predicted MWs of 67KDa, 65KDa and 56KDa, respectively.
IP
1/50 - 1/70.
特記事項
ICC/IF
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 56-67 kDa (predicted molecular weight: 67 kDa).

Based on the sequence analysis by BLAST, ab185942 recognizes three isoforms with predicted MWs of 67KDa, 65KDa and 56KDa, respectively.
IP
1/50 - 1/70.

ターゲット情報

  • 機能

    Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Kiaa0391 antibody
      • KIAA0391 gene antibody
      • Mitochondrial ribonuclease P protein 3 antibody
      • Mitochondrial RNase P protein 3 antibody
      • Mitochondrial RNase P subunit 3 antibody
      • MRPP3 antibody
      • MRRP3_HUMAN antibody
      • PRORP antibody
      • Proteinaceous RNase P antibody
      see all

    画像

    • Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
      Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
      All lanes : Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/5000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 67 kDa

    • Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
      Western blot - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
      Anti-MRPP3 antibody [EPR14321-30] (ab185942) at 1/1000 dilution + U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 67 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
      Immunocytochemistry/ Immunofluorescence - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

      Immunofluorescent analysis of acetone-fixed HeLa cells labeling MRPP3 with ab185942 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

    • Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (ab185942)
      Immunoprecipitation - Anti-MRPP3 antibody [EPR14321-30] (ab185942)

      Western blot analysis of MRPP3 in 293 cell lysate immunoprecipitated using ab185942 at 1/50 dilution (Lane 1).  Lane 2: PBS instead of 293 lysate.  Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.  

    • Anti-MRPP3 antibody [EPR14321-30] (ab185942)
      Anti-MRPP3 antibody [EPR14321-30] (ab185942)

    参考文献 (1)

    ab185942 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185942 は 1 報の論文で使用されています。

    • Tan BG  et al. The human mitochondrial genome contains a second light strand promoter. Mol Cell 82:3646-3660.e9 (2022). PubMed: 36044900

    レビューと Q&A

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