Anti-MRPL48 抗体 [EPR16328] (ab194826)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16328] to MRPL48
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MRPL48 antibody [EPR16328]
MRPL48 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16328] to MRPL48 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, HeLa, PC12, NIH 3T3, Jurkat, Human fetal heart lysate; mouse brain, mouse heart, rat brain and rat heart lysates; Human cervix carcinoma tissue; HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16328 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab194826の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/60.
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IHC-P |
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).
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ICC/IF |
1/150.
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IP |
1/30.
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特記事項 |
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Flow Cyt (Intra)
1/60. |
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa). |
ICC/IF
1/150. |
IP
1/30. |
ターゲット情報
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細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 51642 Human
- Entrez Gene: 52443 Mouse
- Entrez Gene: 293149 Rat
- Omim: 611853 Human
- SwissProt: Q96GC5 Human
- SwissProt: Q8JZS9 Mouse
- Unigene: 503239 Human
- Unigene: 473730 Mouse
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別名
- 39S ribosomal protein L48 antibody
- 39S ribosomal protein L48 mitochondrial antibody
- CGI 118 antibody
see all
画像
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All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL48 antibody [EPR16328] (ab194826)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling MRPL48 with ab194826 at 1/150 dilution. A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab194826. Cytoplasm staining on human cervix carcinoma tissue was observed.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of A431 cells labeling MRPL48 with ab194826 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HepG2 cell line was observed.
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Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilution + Human fetal heart tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
Intracellular Flow Cytometry analysis of HeLa cells labelling MRPL48 (red) with purified ab194826 at dilution of 1/60. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab194826 at 1/30 dilution (lane 1) or PBS control (lane 2)
For Western blot: ab194826 was used at 1/1000 dilution (0.6µg/ml). Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab194826 は 4 報の論文で使用されています。
- Averina OA et al. Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35682734
- Min S et al. MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness. Cell Death Dis 12:1076 (2021). WB ; Human . PubMed: 34772924
- Tong M et al. Comparative pharmacoproteomics reveals potential targets for berberine, a promising therapy for colorectal cancer. Biochem Biophys Res Commun N/A:N/A (2020). PubMed: 32087971
- Laptev I et al. METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue. Nucleic Acids Res 48:8022-8034 (2020). PubMed: 32573735