Anti-MRPL18 抗体 (ab67844)
Key features and details
- Mouse polyclonal to MRPL18
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MRPL18 antibody -
製品の詳細
Mouse polyclonal to MRPL18 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human MRPL18. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
ポジティブ･コントロール
- Human liver tissue lysate, transfected 293T cell lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67844の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa). |
ターゲット情報
-
配列類似性
Belongs to the ribosomal protein L18P family. -
細胞内局在
Mitochondrion. - Information by UniProt
-
参照データベース
- Entrez Gene: 29074 Human
- Omim: 611831 Human
- SwissProt: Q9H0U6 Human
- Unigene: 416998 Human
-
別名
- 39S ribosomal protein L18 antibody
- 39S ribosomal protein L18 mitochondrial antibody
- 39S ribosomal protein L18, mitochondrial precursor antibody
see all
画像
-
Western blot - Anti-MRPL18 antibody (ab67844)Anti-MRPL18 antibody (ab67844) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Western blot - Anti-MRPL18 antibody (ab67844)All lanes : Anti-MRPL18 antibody (ab67844) at 1/500 dilution
Lane 1 : MRPL18 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab67844 は 3 報の論文で使用されています。
- Naesens L et al. GTF3A mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand RNA5SP141. Sci Immunol 7:eabq4531 (2022). PubMed: 36399538
- Chiang JJ et al. Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity. Nat Immunol 19:53-62 (2018). PubMed: 29180807
- Smirnov A et al. Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18. Genes Dev 25:1289-305 (2011). WB ; Human . PubMed: 21685364
