AB67844

Anti-MRPL18 抗体

Name: Anti-MRPL18 抗体 (ab67844) | Abcam
Brand: Abcam Limited
SKU: AB67844
Price: 76000 JPY
Availability: InStock

Anti-MRPL18 antibody

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(3 Publications)

Mouse Polyclonal MRPL18 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MRPL18.

別名を表示する

HSPC071, MRPL18, Large ribosomal subunit protein uL18m, L18mt, MRP-L18

2 Images

Unknown

Western blot - Anti-MRPL18 antibody (AB67844)

All lanes:

Western blot - Anti-MRPL18 antibody (ab67844) at 1/500 dilution

All lanes:

Human liver tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Predicted band size: 21 kDa

Observed band size: 18 kDa

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Western blot - Anti-MRPL18 antibody (AB67844)

All lanes:

Western blot - Anti-MRPL18 antibody (ab67844) at 1/500 dilution

Lane 1:

MRPL18 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondaryantibody at 1/2500 dilution

Predicted band size: 21 kDa

Observed band size: 19 kDa,21 kDa

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Key facts

宿主種

Mouse

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

applications

免疫原

Recombinant Full Length Protein corresponding to Human MRPL18.

Q9H0U6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MRPL18 also known as mitochondrial ribosomal protein L18 is an integral component of the mitochondrial ribosome. This protein has a molecular mass of about 21 kDa. It plays a mechanical role in the translation of mitochondrial mRNA aiding the assembly of protein synthesis machinery. MRPL18 finds expression predominantly in tissues with high energy requirements such as heart and brain.

Biological function summary

MRPL18 is essential for mitochondrial protein biosynthesis. It forms part of the 39S subunit of the mitochondrial ribosome implicating its role in the production of polypeptides catalyzed by mitochondrial DNA. By facilitating efficient translation of genetic material into functional proteins MRPL18 collaborates with other ribosomal proteins and rRNA to maintain mitochondrial function.

Pathways

MRPL18 significantly contributes to oxidative phosphorylation and energy generation pathways. It supports these pathways by translating essential components of the electron transport chain. Closely related proteins like MRPL49 and MRPS16 also participate in these pathways together ensuring effective energy transduction within the cell.

MRPL18 shows associations with mitochondrial diseases such as mitochondrial encephalomyopathy. Impaired function or mutations in MRPL18 can disrupt mitochondrial protein synthesis leading to defective energy metabolism. The target's relationship with proteins like MRPS22 highlights its involvement in disease mechanisms affecting cellular energy homeostasis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Together with thiosulfate sulfurtransferase (TST), acts as a mitochondrial import factor for the cytosolic 5S rRNA. The precursor form shows RNA chaperone activity; is able to fold the 5S rRNA into an import-competent conformation that is recognized by rhodanese (TST). Both the cytoplasmic and mitochondrial forms are able to bind to the helix IV-loop D in the gamma domain of the 5S rRNA.

See full target information MRPL18

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Science immunology 7:eabq4531 PubMed36399538

2022

mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand .

Applications

Unspecified application

Species

Unspecified reactive species

Leslie Naesens,Santoshi Muppala,Dhiraj Acharya,Josephine Nemegeer,Delfien Bogaert,Jung-Hyun Lee,Katrien Staes,Veronique Debacker,Pieter De Bleser,Marieke De Bruyne,Elfride De Baere,Michiel van Gent,GuanQun Liu,Bart N Lambrecht,Jens Staal,Tessa Kerre,Rudi Beyaert,Jonathan Maelfait,Simon J Tavernier,Michaela U Gack,Filomeen Haerynck

Nature immunology 19:53-62 PubMed29180807

2017

Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica J Chiang,Konstantin M J Sparrer,Michiel van Gent,Charlotte Lässig,Teng Huang,Nikolaus Osterrieder,Karl-Peter Hopfner,Michaela U Gack

Genes & development 25:1289-305 PubMed21685364

2011

Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18.

Applications

Species

Human

Alexandre Smirnov,Nina Entelis,Robert P Martin,Ivan Tarassov

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRPL18 抗体

Anti-MRPL18 antibody

Western blot - Anti-MRPL18 antibody (AB67844)

Western blot - Anti-MRPL18 antibody (AB67844)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise