Anti-MRP4 抗体 (ab77184)
Key features and details
- Goat polyclonal to MRP4
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MRP4 antibody
MRP4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MRP4 -
由来種
Goat -
特異性
ab77184 is expected to recognize MRP4 isoform 1 (NP_005836.2) only. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding with sequence CNGQPSTLTIFETAL from the C terminal region of the human MRP4 protein (NP_005836.2)
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ポジティブ･コントロール
- Lysates prepared from rat renal cortex membranes. Human prostate tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab77184 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77184の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 150 kDa).
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 150 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be an organic anion pump relevant to cellular detoxification. -
組織特異性
Widely expressed, with particularly high levels in prostate, but is barely detectable in liver. -
配列類似性
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10257 Human
- Entrez Gene: 239273 Mouse
- Entrez Gene: 170924 Rat
- Omim: 605250 Human
- SwissProt: O15439 Human
- Unigene: 508423 Human
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別名
- ABCC 4 antibody
- ABCC4 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
see all
画像
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Western blot - Anti-MRP4 antibody (ab77184)Anti-MRP4 antibody (ab77184) at 0.5 µg/ml + rat renal cortex membranes at 20 µg
Predicted band size: 150 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody (ab77184)ab77184 (4µg/ml) staining of paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab77184 は 9 報の論文で使用されています。
- Okamoto K et al. Antioxidant effect of ascorbic acid against cisplatin-induced nephrotoxicity and P-glycoprotein expression in rats. Eur J Pharmacol 909:174395 (2021). PubMed: 34332922
- Wang H et al. Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells. Oncol Lett 18:63-71 (2019). PubMed: 31289473
- Dolezelova E et al. High soluble endoglin levels regulate cholesterol homeostasis and bile acids turnover in the liver of transgenic mice. Life Sci 232:116643 (2019). PubMed: 31299237
- Castella B et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). PubMed: 28580927
- Chapy H et al. Blood-brain and retinal barriers show dissimilar ABC transporter impacts and concealed effect of P-glycoprotein on a novel verapamil influx carrier. Br J Pharmacol 173:497-510 (2016). PubMed: 26507673
- Canet MJ et al. Renal xenobiotic transporter expression is altered in multiple experimental models of nonalcoholic steatohepatitis. Drug Metab Dispos 43:266-72 (2015). PubMed: 25488932
- Campia I et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS One 10:e0126159 (2015). WB . PubMed: 25955018
- Meng R et al. SLC35D3 delivery from megakaryocyte early endosomes is required for platelet dense granule biogenesis and is differentially defective in Hermansky-Pudlak syndrome models. Blood 120:404-14 (2012). PubMed: 22611153
- Xu S et al. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. Am J Physiol Gastrointest Liver Physiol 299:G126-35 (2010). ICC/IF . PubMed: 20395535