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AB15603

Anti-MRP2 抗体 [M2III-5]

Anti-MRP2 antibody [M2III-5]

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(14 Publications)

Mouse Monoclonal MRP2 antibody. Suitable for WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Abcc2.

別名を表示する

CMOAT, CMOAT1, CMRP, MRP2, ABCC2, ATP-binding cassette sub-family C member 2, Canalicular multidrug resistance protein, Canalicular multispecific organic anion transporter 1, Multidrug resistance-associated protein 2

1 Images
Western blot - Anti-MRP2 antibody [M2III-5] (AB15603)
  • WB

Lab

Western blot - Anti-MRP2 antibody [M2III-5] (AB15603)

Lanes 1 - 2 : Merged signal (red and green). Green - ab15603 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.

ab15603 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab15603 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MRP2 antibody [M2III-5] (ab15603) at 1/20 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ABCC2 knockout A549 cell line (<a href='/products/cell-lines/human-abcc2-knockout-a549-cell-line-ab261855'>ab261855</a>)

Predicted band size: 174 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

M2III-5

アイソタイプ

IgG2b

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Recombinant Fragment Protein within Rat Abcc2. The exact immunogen used to generate this antibody is proprietary information.

Q63120

エピトープ

ab15603 reacts with an internal epitope of MRP2; a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome.

特異性

This antibody does not cross-react with the human MDR1 P-gp, MRP1, MRP3, or MRP5 gene products.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20 - 1/50", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/20 - 1/50", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.3 Preservative: 0.1% Sodium azide Constituents: Tissue culture supernatant, 0.7% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MRP2 also known as ABCC2 or MRP-2 transporter is an ATP-binding cassette transporter. This protein with a molecular mass of about 190 kDa is widely expressed in tissues such as the liver kidney and intestine. MRP2 plays a role in drug and metabolite transport moving various organic anions across cellular membranes. It locates to the canalicular membrane of hepatocytes where it functions in secreting conjugated bilirubin and drugs into bile.
Biological function summary

MRP2 contributes to cellular detoxification processes. It does not form part of a larger protein complex but works as an independent entity. MRP2 protein actively transports conjugated substances out of cells protecting tissues from potential damage from toxins drugs and other organic anions. This transporter mediates the excretion of glucuronide sulfate and glutathione conjugates playing an important role in phase III of drug metabolism.

Pathways

MRP2 transporter is integral to xenobiotic metabolism. It interconnects with the conjugation pathways that prepare compounds for excretion. The pathway functions with cytochrome P450 enzymes and other related transport proteins like MDR1 facilitating the secretion of detoxified metabolites. MRP2 activity influences pharmacokinetics and pharmacodynamics by modulating drug disposition and excretion.

MRP2 is associated with Dubin-Johnson syndrome and cholestasis. Dubin-Johnson syndrome arises from mutations in the ABCC2 gene leading to defective bilirubin excretion and jaundice. Cholestasis involves impaired bile excretion where MRP2 and possibly related proteins like BSEP play roles in bile salt export. Understanding MRP2 functioning aids in assessing risk and treatment strategies for these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes. Transports a wide variety of conjugated organic anions such as sulfate-, glucuronide- and glutathione (GSH)-conjugates of endo- and xenobiotics substrates (PubMed : 10220572, PubMed : 10421658, PubMed : 11500505, PubMed : 16332456). Mediates hepatobiliary excretion of mono- and bis-glucuronidated bilirubin molecules and therefore play an important role in bilirubin detoxification (PubMed : 10421658). Mediates also hepatobiliary excretion of others glucuronide conjugates such as 17beta-estradiol 17-glucosiduronic acid and leukotriene C4 (PubMed : 11500505). Transports sulfated bile salt such as taurolithocholate sulfate (PubMed : 16332456). Transports various anticancer drugs, such as anthracycline, vinca alkaloid and methotrexate and HIV-drugs such as protease inhibitors (PubMed : 10220572, PubMed : 11500505, PubMed : 12441801). Confers resistance to several anti-cancer drugs including cisplatin, doxorubicin, epirubicin, methotrexate, etoposide and vincristine (PubMed : 10220572, PubMed : 11500505).
See full target information ABCC2
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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 9:18375-18384 PubMed38680328

2024

Ultrasound-Assisted Extraction of (DC.) Koidz. Polysaccharides and the Synergistic Antigastric Cancer Effect in Combination with Oxaliplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Minjie Liang,Yayun Wu,Jimin Sun,Ya Zhao,Lijuan Liu,Ruizhi Zhao,Yan Wang

Biotechnology and bioengineering 119:1018-1032 PubMed34931302

2022

High-throughput production of liver parenchymal microtissues and enrichment of organ-specific functions in gelatin methacrylamide microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Ramesh Pai Roopesh,Senthilkumar Muthusamy,Shiny Velayudhan,Arumugham Sabareeswaran,Pallickaveedu RajanAsari Anil Kumar

Materials today. Bio 13:100182 PubMed34917923

2021

Investigation of the role of the autophagic protein LC3B in the regulation of human airway epithelium cell differentiation in COPD using a biomimetic model.

Applications

Unspecified application

Species

Unspecified reactive species

Shiue-Luen Chen,Hsiao-Chun Chou,Kuan-Chen Lin,Jia-Wei Yang,Ren-Hao Xie,Chong-You Chen,Xin-Yi Liu,Johnson H Y Chung,Guan-Yu Chen

European journal of pharmacology 909:174395 PubMed34332922

2021

Antioxidant effect of ascorbic acid against cisplatin-induced nephrotoxicity and P-glycoprotein expression in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Okamoto,Fumi Kitaichi,Yoshitaka Saito,Hinata Ueda,Katsuya Narumi,Ayako Furugen,Masaki Kobayashi

Physiological reports 7:e14084 PubMed31033251

2019

Acute exercise and brain BACE1 protein content: a time course study.

Applications

Unspecified application

Species

Unspecified reactive species

Alex J Yang,Grant C Hayward,Rebecca E K MacPherson

Physiological reports 6:e13729 PubMed29890051

2018

Evaluation of neuropathological effects of a high-fat high-sucrose diet in middle-aged male C57BL6/J mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bradley J Baranowski,Kirsten N Bott,Rebecca E K MacPherson

Oncology reports 35:2576-82 PubMed26985715

2016

Loss of Runt-related transcription factor 3 induces resistance to 5-fluorouracil and cisplatin in hepatocellular carcinoma.

Applications

IHC

Species

Unspecified reactive species

Junro Kataoka,Hidenori Shiraha,Shigeru Horiguchi,Hiroaki Sawahara,Daisuke Uchida,Teruya Nagahara,Masaya Iwamuro,Hiroki Morimoto,Yasuto Takeuchi,Kenji Kuwaki,Hideki Onishi,Shinichiro Nakamura,Akinobu Takaki,Kazuhiro Nouso,Takahito Yagi,Kazuhide Yamamoto,Hiroyuki Okada

Drug metabolism and disposition: the biological fate of chemicals 40:450-60 PubMed22112382

2011

Molecular mechanism of altered ezetimibe disposition in nonalcoholic steatohepatitis.

Applications

WB

Species

Rat

Rhiannon N Hardwick,Craig D Fisher,Stephanie M Street,Mark J Canet,Nathan J Cherrington

Drug metabolism and disposition: the biological fa 39:2395-402 PubMed21878559

2011

Variations in ATP-binding cassette transporter regulation during the progression of human nonalcoholic fatty liver disease.

Applications

WB

Species

Human

Rhiannon N Hardwick,Craig D Fisher,Mark J Canet,George L Scheffer,Nathan J Cherrington

Biochemical pharmacology 81:178-84 PubMed20868650

2010

LPS-induced dissociation of multidrug resistance-associated protein 2 (Mrp2) and radixin is associated with Mrp2 selective internalization in rats.

Applications

WB, IHC-Fr

Species

Rat, Rat

Junjiro Saeki,Shuichi Sekine,Toshiharu Horie
View all publications
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