Anti-MRP2 抗体 [EPR10997(2)] (ab187644)
![Western blot - Anti-MRP2 antibody [EPR10997(2)] (ab187644)](https://www.abcam.co.jp/ps/products/187/ab187644/Images/ab187644-345654-Anti-MRP2-antibody-EPR109972-western-blot-human.jpg "Western blot - Anti-MRP2 antibody [EPR10997(2)] (ab187644)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10997(2)] to MRP2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MRP2 antibody [EPR10997(2)]
MRP2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10997(2)] to MRP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab112272) -
ポジティブ･コントロール
- Human Liver tissue; Human fetal liver lysate; HepG2 and A549 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10997(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187644の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 174 kDa.Can be blocked with Recombinant Human MRP2 protein (ab112272).
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 174 kDa.Can be blocked with Recombinant Human MRP2 protein (ab112272). |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. -
組織特異性
Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. -
関連疾患
Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function. -
配列類似性
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1244 Human
- Omim: 601107 Human
- SwissProt: Q92887 Human
- Unigene: 368243 Human
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別名
- ABC30 antibody
- abcC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
see all
画像
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Western blot - Anti-MRP2 antibody [EPR10997(2)] (ab187644)All lanes : Anti-MRP2 antibody [EPR10997(2)] (ab187644) at 1/1000 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 174 kDaLanes 1 - 4: Merged signal (red and green). Green - ab187644 observed at 210 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab187644 was shown to recognize MRP2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab187644 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10997(2)] (ab187644)](https://www.abcam.co.jp/ps/products/187/ab187644/Images/ab187644-220773-anti-mrp2-antibody-epr109972-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP2 antibody [EPR10997(2)] (ab187644)Immunohistochemistry analysis of paraffin-embedded Human liver tissue labeling MRP2 with ab187644 at 1/1000 dilution. A prediluted HRP polymer for Rabbit IgG used as secondary antibody. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-MRP2 antibody [EPR10997(2)] (ab187644)](https://www.abcam.co.jp/ps/products/187/ab187644/Images/ab187644-220772-anti-mrp2-antibody-epr109972-western-blot.jpg)
Western blot - Anti-MRP2 antibody [EPR10997(2)] (ab187644)All lanes : Anti-MRP2 antibody [EPR10997(2)] (ab187644) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : A549 lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 174 kDa -
![Anti-MRP2 antibody [EPR10997(2)] (ab187644)](https://www.abcam.co.jp/ps/products/187/ab187644/Images/ab187644-4-benefits-of-recombinant-antibodies.png)
Anti-MRP2 antibody [EPR10997(2)] (ab187644)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab187644 は 2 報の論文で使用されています。
- Aiba Y et al. Increased expression and altered localization of cathepsin Z are associated with progression to jaundice stage in primary biliary cholangitis. Sci Rep 8:11808 (2018). PubMed: 30087368
- Sakai Y et al. Time-dependent structural and functional characterization of subcutaneous human liver tissue. J Tissue Eng Regen Med 12:2287-2298 (2018). PubMed: 30353697