Anti-MRGX1 抗体 (ab77519)
Key features and details
- Rabbit polyclonal to MRGX1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MRGX1 antibody -
製品の詳細
Rabbit polyclonal to MRGX1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived from internal sequence of human GPCR MRGX1.
-
ポジティブ･コントロール
- Extracts from MCF-7 cells. HepG2 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77519の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
|
|
| ICC/IF |
1/500 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
|
ICC/IF
1/500 - 1/1000. |
ターゲット情報
-
機能
Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22)(PubMed:26582731). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells. G(alpha)q proteins are involved in the calcium-signaling pathway. Activated by the antimalarial drug, chloroquine. May mediate chloroquine-induced itch, in a histamine-independent manner. -
組織特異性
Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Mas subfamily. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 259249 Human
- Omim: 607227 Human
- SwissProt: Q96LB2 Human
- Unigene: 711459 Human
-
別名
- GPCR antibody
- MAS related gene MRGX1 antibody
- MAS related GPR member X1 antibody
see all
画像
-
Western blot - Anti-MRGX1 antibody (ab77519)All lanes : Anti-MRGX1 antibody (ab77519) at 1/500 dilution
Lane 1 : Extracts from MCF-7 cells
Lane 2 : Extracts from MCF-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MRGX1 antibody (ab77519)Immunofluorescence analysis of GPCR MRGX1 in HepG2 cells using ab77519, at 1/500 dilution, in the absence or presence of immunising peptide
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab77519 は論文での使用が確認できていません。