AB178405

Anti-MRGX 抗体 [EPR9775(2)]

Anti-MRGX antibody [EPR9775(2)]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MRGX antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

別名を表示する

KIAA0026, MRGX, MORF4L2, Mortality factor 4-like protein 2, MORF-related gene X protein, Protein MSL3-2, Transcription factor-like protein MRGX

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (AB178405)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human ovarian carcinoma tissue labeling MRGX with ab178405 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast tissue labeling MRGX with ab178405 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (AB178405)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Immunofluorescent analysis of HeLa cells labeling MRGX with ab178405 at 1/50 dilution.

Supplier Data

Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRGX immunoprecipitated using ab178405 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)

Predicted band size: 32 kDa

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Supplier Data

Western blot - Anti-MRGX antibody [EPR9775(2)] (AB178405)

All lanes:

Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

U-87 MG cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 32 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9775(2)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MRGX also known as Mas-related G protein-coupled receptor member X functions as a G protein-coupled receptor (GPCR) involved in sensing environmental stimuli and transducing signals inside cells. Researchers find this receptor in sensory neurons within the dorsal root ganglia and trigeminal ganglia. MRGX weighs approximately 37 kDa and plays a role in pain perception and modulation by interacting with various ligands. Understanding its mechanical function helps in exploring its wide applications in sensory biology.

Biological function summary

This receptor contributes to the modulation of nociceptive signals and influences pain sensation and response. MRGX does not form part of a complex; it functions independently to modulate pain pathways by interacting with peptides and other small molecules. It also regulates calcium ion concentrations within neurons which is essential for neurotransmission and signal propagation. The interaction of MRGX with these elements highlights its significant contribution to the sensory regulatory processes.

Pathways

Research associates MRGX with pain perception and sensory transmission pathways. It intersects with the neuropeptide signaling pathway influencing proteins such as substance P and calcitonin gene-related peptide (CGRP) both key players in pain signal pathways. MRGX modulates the activity of these signaling molecules affecting how the body interprets and reacts to painful stimuli. This receptor's involvement in these pathways demonstrates its impact on the physiological responses to pain.

MRGX is linked to conditions such as neuropathic pain and inflammatory pain. Alterations in MRGX function or expression can influence the severity of pain-related phenomena implicating it in these conditions. The association of MRGX with proteins like TRPV1 and Nav1.8 which are involved in pain pathways further solidifies its connection to these disorders. Understanding these interactions offers insights into potential therapeutic avenues for treating chronic pain syndromes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also a component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones.

See full target information MORF4L2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neuron 99:1155-1169.e9 PubMed30174115

2018

Muskelin Coordinates PrP Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Frank F Heisler,Yvonne Pechmann,Ines Wieser,Hermann C Altmeppen,Leonhard Veenendaal,Mary Muhia,Michaela Schweizer,Markus Glatzel,Susanne Krasemann,Matthias Kneussel

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRGX 抗体 [EPR9775(2)]

Anti-MRGX antibody [EPR9775(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (AB178405)

Western blot - Anti-MRGX antibody [EPR9775(2)] (AB178405)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise