Anti-Mre11 抗体 [EPR3471] (ab109623)
![Western blot - Anti-Mre11 antibody [EPR3471] (ab109623)](https://www.abcam.co.jp/ps/products/109/ab109623/Images/ab109623-122569-anti-mre11-antibody-epr3471-western-blot.jpg "Western blot - Anti-Mre11 antibody [EPR3471] (ab109623)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3471] to Mre11
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Mre11 antibody [EPR3471]
Mre11 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3471] to Mre11 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- K562, HT-29, Jurkat, and HeLa whole cell lysate (ab150035). Human cervical carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3471 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109623の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 81 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 81 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.
ターゲット情報
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機能
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
関連疾患
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. -
配列類似性
Belongs to the MRE11/RAD32 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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参照データベース
- Entrez Gene: 4361 Human
- Entrez Gene: 17535 Mouse
- Entrez Gene: 64046 Rat
- Omim: 600814 Human
- SwissProt: P49959 Human
- SwissProt: Q61216 Mouse
- SwissProt: Q9JIM0 Rat
- Unigene: 192649 Human
see all -
別名
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
see all
画像
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Western blot - Anti-Mre11 antibody [EPR3471] (ab109623)All lanes : Anti-Mre11 antibody [EPR3471] (ab109623) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HT-29 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 81 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR3471] (ab109623)](https://www.abcam.co.jp/ps/products/109/ab109623/Images/ab109623-122568-anti-mre11-antibody-epr3471-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR3471] (ab109623)ab109623 at 1/50 dilution staining Mre11 in paraffin-embedded Human cervical carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-Mre11 antibody [EPR3471] (ab109623)](https://www.abcam.co.jp/ps/products/109/ab109623/Images/ab109623-3-benefits-of-recombinant-antibodies.png)
Anti-Mre11 antibody [EPR3471] (ab109623)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab109623 は 6 報の論文で使用されています。
- Feng YL et al. Proximal binding of dCas9 at a DNA double strand break stimulates homology-directed repair as a local inhibitor of classical non-homologous end joining. Nucleic Acids Res 51:2740-2758 (2023). PubMed: 36864759
- Huang M et al. PARP1 negatively regulates transcription of BLM through its interaction with HSP90AB1 in prostate cancer. J Transl Med 21:445 (2023). PubMed: 37415147
- Ye C et al. High glucose induces the proliferation of prostatic cells via downregulating MRE11. Int J Mol Med 41:3105-3114 (2018). PubMed: 29532862
- Vadnais C et al. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. Nat Commun 9:1418 (2018). PubMed: 29651020
- Tsukagoshi M et al. Overexpression of karyopherin-a2 in cholangiocarcinoma correlates with poor prognosis and gemcitabine sensitivity via nuclear translocation of DNA repair proteins. Oncotarget 8:42159-42172 (2017). PubMed: 28178675
- Altan B et al. High Expression of MRE11-RAD50-NBS1 Is Associated with Poor Prognosis and Chemoresistance in Gastric Cancer. Anticancer Res 36:5237-5247 (2016). PubMed: 27798884