Anti-MRC2/ENDO180 抗体 (ab70132)
Key features and details
- Rabbit polyclonal to MRC2/ENDO180
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MRC2/ENDO180 antibody
MRC2/ENDO180 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MRC2/ENDO180 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab70132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70132の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) | |
| IHC-P | (1) | |
| ICC/IF | (1) |
| 特記事項 |
|---|
Flow Cyt: 1/5 (Abreview).
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 167 kDa (predicted molecular weight: 167 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs). -
組織特異性
Ubiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney. -
配列類似性
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
ドメイン
C-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner.
Fibronectin type-II domain mediates collagen-binding.
Ricin B-type lectin domain contacts with the second C-type lectin domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9902 Human
- Omim: 612264 Human
- SwissProt: Q9UBG0 Human
- Unigene: 7835 Human
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別名
- C-type lectin domain family 13 member E antibody
- C-type mannose receptor 2 antibody
- CD 280 antibody
see all
画像
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Western blot - Anti-MRC2/ENDO180 antibody (ab70132)All lanes : Anti-MRC2/ENDO180 antibody (ab70132) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 167 kDa
Observed band size: 167 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MRC2/ENDO180 antibody (ab70132)ab70132, at a 1/500 dilution, staining MRC2/ENDO180 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody (ab70132)ab70132, at a 1/50 dilution, staining MRC2/ENDO180 in paraffin embedded human brain tissue by Immunohistochemistry, in the absence or presence of the immunising peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody (ab70132)This image is courtesy of an anonymous Abreviewab70132 staining MRC2/ENDO180 in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Inhibitor CM for 4 minutes at 37°C; antigen retrieval was by heat mediation in Tris/Borate/EDTA buffer pH 8. Samples were incubated with primary antibody (1/50 ) for 32 minutes at 37°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab70132 は 12 報の論文で使用されています。
- Wang Y et al. Comprehensive Surfaceome Profiling to Identify and Validate Novel Cell-Surface Targets in Osteosarcoma. Mol Cancer Ther 21:903-913 (2022). PubMed: 35312779
- Yang X et al. Elevated LINC00909 Promotes Tumor Progression of Ovarian Cancer via Regulating the miR-23b-3p/MRC2 Axis. Oxid Med Cell Longev 2021:5574130 (2021). PubMed: 34336102
- Wu BM et al. Margatoxin mitigates CCl4-induced hepatic fibrosis in mice via macrophage polarization, cytokine secretion and STAT signaling. Int J Mol Med 45:103-114 (2020). PubMed: 31746414
- Wohlfahrt T et al. PU.1 controls fibroblast polarization and tissue fibrosis. Nature 566:344-349 (2019). PubMed: 30700907
- Hsiung S et al. Hyperglycemia does not affect tissue repair responses in shear stress-induced atherosclerotic plaques in ApoE-/- mice. Sci Rep 8:7530 (2018). PubMed: 29760458