Anti-MPP5/Pals1 抗体 (ab20511)
Key features and details
- Goat polyclonal to MPP5/Pals1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MPP5/Pals1 antibody
MPP5/Pals1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MPP5/Pals1 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human MPP5/Pals1 aa 174-187 (N terminal).
Sequence:NKASPPFPLISNAQ
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ポジティブ・コントロール
- Human Brain lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20511の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton. -
組織特異性
Expressed in the retina (at protein level). -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain. -
ドメイン
The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
The PDZ domain binds to the C-terminus of SC6A1. -
細胞内局在
Cell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon. - Information by UniProt
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参照データベース
- Entrez Gene: 64398 Human
- Omim: 606958 Human
- SwissProt: Q8N3R9 Human
- Unigene: 652312 Human
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別名
- FLJ12615 antibody
- MAGUK p55 subfamily member 5 antibody
- Membrane protein palmitoylated 5 antibody
see all
画像
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Anti-MPP5/Pals1 antibody (ab20511) at 1 µg/ml + Human brain lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab20511 は論文での使用が確認できていません。