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AB108528

Anti-MPP1 抗体 [EPR5865]

Anti-MPP1 antibody [EPR5865]

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(1 Publication)

Rabbit Recombinant Monoclonal MPP1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

DXS552E, EMP55, MPP1, 55 kDa erythrocyte membrane protein, p55

2 Images
Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)
  • WB

Lab

Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)

Lanes 1-4 : Merged signal (red and green). Green - ab108528 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

ab108528 Anti-MPP1 antibody [EPR5865] was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267248 (knockout cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab108528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MPP1 antibody [EPR5865] (ab108528) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

MPP1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human MPP1 knockout HEK-293T cell line (ab267248)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)
  • WB

Unknown

Western blot - Anti-MPP1 antibody [EPR5865] (AB108528)

All lanes:

Western blot - Anti-MPP1 antibody [EPR5865] (ab108528) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa,21 kDa,52 kDa

Observed band size: 30 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-MPP1 antibody [EPR5865] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5865

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The MPP1 protein also known as Membrane Palmitoylated Protein 1 acts as a part of the membrane-associated guanylate kinase (MAGUK) family. This protein has a molecular mass of around 55 kDa. MPP1 expresses mainly in erythrocytes the cells that carry oxygen in the blood. Functionally it plays a role in maintaining cell shape and stability by contributing to the cytoskeletal structure. It localizes at the plasma membrane and interacts with other proteins to fulfill its functions.
Biological function summary

In various cell types MPP1 supports cell adhesion and communication. As a complex-forming protein MPP1 interacts with spectrin and actin contributing to the structural organization of cell membranes. MPP1 regulates interactions within the cellular environment influencing cellular signaling and mechanical integrity. By establishing these connections it helps maintain cell shape and facilitate cell signaling processes.

Pathways

Several interactions actively involve MPP1. Within the pathways regulating cell shape MPP1 contributes to the RhoA signaling pathway important for cytoskeletal organization. Furthermore MPP1 closely works with proteins like Ezrin to support plasma membrane stability and intracellular communication. These interactions are significant for maintaining normal cellular junctions and morphology.

MPP1 links to specific conditions with impacts on erythrocyte function. It has been implicated in hereditary spherocytosis a disorder affecting red blood cell stability and causing anemia. MPP1 also interacts with the protein Ankyrin an important component in maintaining erythrocyte membrane structure. These associations highlight the importance of MPP1 in blood-related diseases marking it as an important target for understanding membrane disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity).
See full target information MPP1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:1547 PubMed29146910

2017

An unexpected protein interaction promotes drug resistance in leukemia.

Applications

WB

Species

Human

Aaron Pitre,Yubin Ge,Wenwei Lin,Yao Wang,Yu Fukuda,Jamshid Temirov,Aaron H Phillips,Jennifer L Peters,Yiping Fan,Jing Ma,Amanda Nourse,Chandrima Sinha,Hai Lin,Richard Kriwacki,James R Downing,Tanja A Gruber,Victoria E Centonze,Anjaparavanda P Naren,Taosheng Chen,John D Schuetz
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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