Anti-Mov10 抗体 (ab80613)
Key features and details
- Rabbit polyclonal to Mov10
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Mov10 antibody
Mov10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mov10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibody was affinity purified using an epitope specific to MOV10 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80613の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa.
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| IP |
Use at 6 µg/mg of lysate.
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa. |
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IP
Use at 6 µg/mg of lysate. |
ターゲット情報
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関連性
MOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 457099 Chimpanzee
- Entrez Gene: 523206 Cow
- Entrez Gene: 483219 Dog
- Entrez Gene: 101128877 Gorilla
- Entrez Gene: 4343 Human
- Entrez Gene: 17454 Mouse
- Entrez Gene: 100458347 Orangutan
- Omim: 610742 Human
see all -
別名
- DKFZp667O1423 antibody
- FLJ32791 antibody
- fSAP113 antibody
see all
画像
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Western blot - Anti-Mov10 antibody (ab80613)Lane 1 : Anti-Mov10 antibody (ab80613) at 1 µg/ml
Lanes 2-5 : Anti-Mov10 antibody (ab80613) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Mov10 antibody (ab80613)Immunoprecipitation of HeLa whole cell lysate using ab80613 at 6µg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab80613 は 13 報の論文で使用されています。
- Wang L et al. MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity. mBio 12:e0131621 (2021). PubMed: 34517762
- Yang H et al. LINC00667 promotes the proliferation, migration, and pathological angiogenesis in non-small cell lung cancer through stabilizing VEGFA by EIF4A3. Cell Biol Int 44:1671-1680 (2020). PubMed: 32281700
- Shah R et al. Single-cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection. J Vis Exp N/A:N/A (2020). PubMed: 33191939
- Liu D et al. Effect of P-body component Mov10 on HCV virus production and infectivity. FASEB J N/A:N/A (2020). PubMed: 32496609
- Liu T et al. The MOV10 helicase restricts hepatitis B virus replication by inhibiting viral reverse transcription. J Biol Chem 294:19804-19813 (2019). PubMed: 31722967