Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)
![Western blot - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)](https://www.abcam.co.jp/ps/products/181/ab181452/Images/ab181452-211171-anti-adfp-antibody-2c5h8-western-blot.jpg "Western blot - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)")
Key features and details
- Mouse monoclonal [2C5H8] to Perilipin-2
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Mouse monoclonal [2C5H8] to Perilipin-2
Perilipin-2 一次抗体 製品一覧 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Cow, Pig
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免疫原
Recombinant fragment corresponding to Human Perilipin-2 aa 250 to the C-terminus. Expressed in E. coli.
Database link: Q99541 -
ポジティブ･コントロール
- Perilipin-2 recombinant protein; Perilipin-2 transfected HEK293 cell lysate; HepG2 cells and cell lysate; Human rectum cancer tissue; Human bladder cancer tissue.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR197175-12 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% stabilization buffer (amino acid =85% pH(1% water solution) =7.0~7.5, Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1) -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
2C5H8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181452の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/1000.
|
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| WB |
1/500 - 1/2000.
|
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| ICC/IF |
1/200 - 1/1000.
|
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| Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/1000. |
|
WB
1/500 - 1/2000. |
|
ICC/IF
1/200 - 1/1000. |
|
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May be involved in development and maintenance of adipose tissue. -
組織特異性
Milk lipid globules. -
配列類似性
Belongs to the perilipin family. -
翻訳後修飾
Acylated; primarily with C14, C16 and C18 fatty acids. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 280981 Cow
- Entrez Gene: 123 Human
- Omim: 103195 Human
- SwissProt: Q9TUM6 Cow
- SwissProt: Q99541 Human
- SwissProt: Q4PLW0 Pig
- Unigene: 3416 Human
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別名
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
画像
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Western blot - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452) at 1/500 dilution + HepG2 cell lysate
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![Western blot - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)](https://www.abcam.co.jp/ps/products/181/ab181452/Images/ab181452-211169-anti-adfp-antibody-2c5h8-western-blot.jpg)
Western blot - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)All lanes : Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Perilipin-2-transfected HEK293 cell lysate -
![Western blot - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)](https://www.abcam.co.jp/ps/products/181/ab181452/Images/ab181452-211168-anti-adfp-antibody-2c5h8-western-blot.jpg)
Western blot - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452) at 1/500 dilution + Perilipin-2 recombinant protein -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)](https://www.abcam.co.jp/ps/products/181/ab181452/Images/ab181452-211167-anti-adfp-antibody-2c5h8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Perilipin-2 with ab181452 at 1/200 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)](https://www.abcam.co.jp/ps/products/181/ab181452/Images/ab181452-211166-anti-adfp-antibody-2c5h8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Perilipin-2 with ab181452 at 1/200 dilution.
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![Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)](https://www.abcam.co.jp/ps/products/181/ab181452/Images/ab181452-211165-anti-adfp-antibody-2c5h8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)Immunofluorescent analysis of HepG2 cells labeling Perilipin-2 with ab181452 (green) at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye.
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![Flow Cytometry - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)](https://www.abcam.co.jp/ps/products/181/ab181452/Images/ab181452-211164-anti-adfp-antibody-2c5h8-flow-cytometry.jpg)
Flow Cytometry - Mouse monoclonal [2C5H8] to Perilipin-2 (ab181452)Flow cytometric analysis of HepG2 cells labeling Perilipin-2 with ab181452 (green) at 1/200 dilution, and negative control (red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab181452 は 4 報の論文で使用されています。
- He Y et al. Lipid Droplet-Related PLIN2 in CD68+ Tumor-Associated Macrophage of Oral Squamous Cell Carcinoma: Implications for Cancer Prognosis and Immunotherapy. Front Oncol 12:824235 (2022). PubMed: 35372038
- Su W et al. Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice. Nat Commun 13:6577 (2022). PubMed: 36323699
- Yuan Y et al. Mitochondrial E3 ubiquitin ligase 1 promotes autophagy flux to suppress the development of clear cell renal cell carcinomas. Cancer Sci 110:3533-3542 (2019). PubMed: 31489722
- Carrillo M et al. Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging. Medicine (Baltimore) 97:e12392 (2018). PubMed: 30278515