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Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype control (ab18421)

Q&A (1)References (5)

Conjugates logo Related conjugates and formulations

FITC

製品の概要

  • 製品名

    Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype control
    Mouse アイソタイプ・コントロール 製品一覧

  • 特異性

    The MG2b-57 immunoglobulin (ab18421) reacts with KLH. The MG2b-57 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

  • アプリケーション

    適用あり: IP, WB, IHC-Fr, IHC-P, Flow Cytmore details

  • 免疫原

    Trinitrophenol + KLH

  • 特記事項

    ab18421 MG2b-57 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    The immunoglobulin was purified by affinity chromatography.

  • アイソタイプ・コントロール 備考

    ab18421 MG2b-57 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MG2b-57

  • アイソタイプ

    IgG2b

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18421の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Surface or intracellular staining.
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Surface or intracellular staining.

参考文献 (5)

ab18421 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18421 は 5 報の論文で使用されています。

  • Liu C  et al. The mechanosensitive lncRNA Neat1 promotes osteoblast function through paraspeckle-dependent Smurf1 mRNA retention. Bone Res 10:18 (2022). PubMed: 35210394
  • Mermoud L  et al. IL-38 orchestrates proliferation and differentiation in human keratinocytes. Exp Dermatol 31:1699-1711 (2022). PubMed: 35833307
  • Zhao X  et al. Human Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus. Stem Cell Reports 16:493-504 (2021). PubMed: 33626333
  • D'Onofrio N  et al. MicroRNA-33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients. J Cell Physiol 235:1438-1452 (2020). PubMed: 31294459
  • Mercado-Matos J  et al. Differential involvement of the microtubule cytoskeleton in insulin receptor substrate 1 (IRS-1) and IRS-2 signaling to AKT determines the response to microtubule disruption in breast carcinoma cells. J Biol Chem 292:7806-7816 (2017). PubMed: 28320862

レビューと Q&A

Q&A
質問を送る

Question

Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. As proof of concept, I’d like to detect known isotype control samples using the pair of antibodies that I have. Now, I’m interested in ab18421 ([MG2b-57]) and ab18469 ([MPC-11]). Which one do you recommend? Are both isotype control samples recognized by the antibodies that I have? Another question I’d like to ask is what is the meaning of clone numbers like MG2b-1 and MG2b-2? Can I know the epitope information from these numbers? Thank you.

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Answer

Thank you for your enquiry.


Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

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