Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
![Immunocytochemistry/ Immunofluorescence - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)](https://www.abcam.co.jp/ps/products/170/ab170192/Images/ab170192-169997-mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-immunocytochemistry-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)")
製品の概要
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製品名
Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control -
特異性
ab170192, mouse IgG2b Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
アプリケーション
適用あり: Flow Cyt, ICC/IF, ELISAmore details -
特記事項
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
This product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
モノクローナル -
クローン名
7E10G10 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170192の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 µg/ml.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Flow Cyt
Use a concentration of 1 µg/ml. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
画像
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Immunocytochemistry/ Immunofluorescence - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Immunofluorscent analysis of NIH 3T3, COS7, H9C2 and HeLa cells using ab170192 at 1µg/ml (top); no primary antibody negative control (middle), and ab14705 as a positive control (bottom). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)](https://www.abcam.co.jp/ps/products/170/ab170192/Images/ab170192-171552-mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-flow-cytometry.jpg)
Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.
The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![ELISA - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)](https://www.abcam.co.jp/ps/products/170/ab170192/Images/ab170192-170000-mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-elisa.jpg)
ELISA - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mLab170192 and detecting with Alexa Fluor® conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab170192 は 6 報の論文で使用されています。
- Li X et al. Pantothenate Kinase 4 Governs Lens Epithelial Fibrosis by Negatively Regulating Pyruvate Kinase M2-Related Glycolysis. Aging Dis 14:1834-1852 (2023). PubMed: 37196116
- Hornung RS et al. Sigma-1 receptors and progesterone metabolizing enzymes in nociceptive sensory neurons of the female rat trigeminal ganglia: A neural substrate for the antinociceptive actions of progesterone. Mol Pain 18:17448069211069255 (2022). PubMed: 35040378
- Liu B et al. Sodium iodate induces ferroptosis in human retinal pigment epithelium ARPE-19 cells. Cell Death Dis 12:230 (2021). PubMed: 33658488
- Andrews JM et al. Loss of synergistic transcriptional feedback loops drives diverse B-cell cancers. EBioMedicine 71:103559 (2021). PubMed: 34461601
- Cao Y et al. LL-37 promotes neutrophil extracellular trap formation in chronic rhinosinusitis with nasal polyps. Clin Exp Allergy 49:990-999 (2019). PubMed: 31046155
- Strieder-Barboza C et al. Advanced glycation end-products regulate extracellular matrix-adipocyte metabolic crosstalk in diabetes. Sci Rep 9:19748 (2019). PubMed: 31875018